EVALUATION OF ANTIGEN-BASED ENZYME IMMUNO-ASSAY IN REFERENCE TO DIRECT MICROSCOPY FOR THE DIAGNOSIS OF ENTAMOEBA HISTOLYTICA IN STOOL SAMPLES | ||
| AL-TAQANI | ||
| Article 1, Volume 27, Issue 2, April 2014, Pages 0-0 | ||
| Authors | ||
| Nada M. Al-Basheer; Ilham A. Majeed; Huda M. Jawad | ||
| Abstract | ||
| In the present study, we have been used TechLab Entamoeba histolytica II Enzyme Linked Immune Sorbent Assay (ELISA) test for detection of Entamoeba histolytica and comparing it with microscopic examination. A total of 80 stool samples were collected from patients with intestinal amoebae, including 47 males and 33 females, the age between (1-60) years. The stool samples were examined by direct microscopy ,then we investigated E. histolytica specific adhesin antigen in stool samples using ELISA( TechLab E. histolytica II test , United States of America USA). Out of 80 stool samples examined microscopically 65 were infected with intestinal amoebae. The examination of the 65 (81.3%) positive samples by using TechLab E. histolytica II ELISA test revealed that 13 (16.3%) were positive for E. histolytica while 2 (2.5 %) positive result using TechLab E. histolytica II ELISA kit was detected among the15 (18.8 %) microscopically negative samples. The study shows the majority of intestinal protozoa occur in males 36 (45.0%) and females 29 (36.3%), as well in E. histolytica high percentage were record in males 9 (11.3%) and in females 6 (7.5%), the results shows the highest percentage of intestinal protozoa as well E. histolytica at age group (30-39) (22.5%) (7.5%) respectively | ||
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