SPECTROPHOTOMETRIC METHOD FOR QUANTITATIVE DETERMINATION OF ADRENALINE IN PHARMACEUTICAL DOSAGE FORM USING METHYLTHIAZOLYLDIPHENYLTETRAZOLIUM- BROMIDE (MTT) | ||
| AL-TAQANI | ||
| Article 1, Volume 27, Issue 2, April 2014, Pages 0-0 | ||
| Authors | ||
| a N. Al-Sabha; ; Thabit S.Al-Ghabsha; Saladdin M.AL-Talib | ||
| Abstract | ||
| A sensitive and simple spectrophotometric method is developed for the determination of adrenaline. The method is based on the reaction of adrenaline with methylthiazolyldiphenyl- tetrazolium- bromide (MTT) in basic medium and the increase of reagent's color intensity is measured at 536 nm against reagent blank. Beers law was valid over the concentration range of 0.1 – 3.0μg.ml-1 with molar absorptivity of 1.179 × 105l.mol-1.cm-1, and Sandell sensitivity of 0.00156 μg. cm-2. The relative error is less than + 3%, and the relative standard deviation of better than + 1.8% depending on the concentration. The proposed method was successfully applied to the assay of the adrenaline in dosage form. | ||
| Keywords | ||
| Spectrophotometry; Adrenaline; Methylthiazolyldiphenyl; tetrazoliumbromide | ||
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