GENETIC STUDY OF RESISTANT PSEUDOMONAS AERUGINOSA ISOLATED FROM DIFFERENT CLINICAL SOURCES TO IMIPENEM | ||
| AL-TAQANI | ||
| Article 1, Volume 27, Issue 2, April 2014, Pages 0-0 | ||
| Authors | ||
| Alaa Salim Hamzah; Adnan Kamil Shabeeb; Riad. A. Dellol | ||
| Abstract | ||
| The antibiotic resistance is now a major clinical problem all over the world . In this study eighty swabs were collected from two different clinical sources; forty samples from wound infections and forty from otitis infections. Out of (23) Pseudomonas aeruginosa isolates there were (14) isolates from wound infections and (9) isolates from otitis infections. There were (5) isolates resistant to imipenem. The plasmid DNA were studied to these five isolates. The primary step were DNA extraction to showing DNA plasmid. The secondary step was curing DNA plasmid with acridin orange by using different concentrations. The results showed that the isolates lost DNA plasmid in 512μg/ml of acridin orange and disappear resistance to the imipenem. The genes that encoded to the resistance Pseudomonas aeruginosa to imipenem may be located on plasmid .These character may led to distribution of resistance to other bacteria by bacterial conjucation. | ||
| Keywords | ||
| Resistant Pseudomonas aeruginosa to Imipenem; Otitis infections; Wound infections | ||
|
Statistics Article View: 135 PDF Download: 15 |
||