The synergistic effect of some antioxidants added to Tris extender on semen freezability of Holstein bulls following different cryopreservation periods | ||
Al-Anbar Journal of Veterinary Sciences | ||
Article 1, Volume 10, Issue 1, June 2017, Pages 1-9 | ||
Authors | ||
T. A. Abdulkareem; K. H. Sultan; M. S. Noon; F. F. Ibrahim; M. A. Haydar; W. Y. Latef | ||
Abstract | ||
This study was undertaken to investigate the synergistic influence of adding some antioxidants to Tris extender on semen freezability of Holstein bulls following different cryopreservation periods (48 h, 1 and 2 months post cryopreservation, PC). This experiment was executed at the Artificial Insemination Department, belong to the Animal Resources Directorate, Ministry of Agriculture in Abu-Ghraib region (25 Km west of Baghdad) during the period from 15th November 2015 to 30th March 2016. Semen was collected via artificial vagina in one ejaculate per bull per week for the 6-week experimental period. Pooled semen was equally divided into five groups using Tris extender. Combinations of vitamin C + carnitine (5 and 7.5 mm/ ml, respectively; A2), vitamin E + coenzyme Q10 (0.2 and 0.5 mm/ ml, respectively; A3), vitamin E + α-lipoic acid (0.2 and 0.5 mm/ ml, respectively; A4) and carnitine + catalase (7.5 mm and 100 IU / ml, respectively; A5) were added to Tris extender and comparisons in response were made with the control group (Tris extender, A1). Greater (P≤0.01) semen freezability was noticed in the A5 group (90.00 ± 5.16%) as compared with the A1, A2 and A4 groups. However, the differences between A5 and A3 (71.66 ± 6.54%) groups, 46 h PC lacked significance. On the other hand, there were no significant differences among A1, A2 and A4 groups in this character. Moreover, the semen freezability did not significantly differ among the preservation periods within each group, in spite of the numerical decreasing percentages at 1 and 2 months PC as compared with 48 h PC. In conclusion, the synergistic effect of adding some antioxidant combinations had improved semen freezability of Holstein bulls, which will positively reflect in enhancing bull’s fertility and owner’s economic returns consequently | ||
Keywords | ||
Antioxidants; semen freezability; Holstein bulls | ||
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