Study on Skeletal muscles during myotoxicity induced by (Bupivacaine) in experimental rats ( Rattus norvegicus). | ||
| Misan Journal of Acodemic Studies | ||
| Article 1, Volume 15, Issue 29, June 2016, Pages 173-199 | ||
| Authors | ||
| Mohammed k. Hassani; Maha K. Ibrahim | ||
| Abstract | ||
| Summary Recent study was aimed to identified the effect of local anaesthetic drugs , Bupivacaine ( Bpvc) injection into the skeletal muscles to induced myotoxicity , So muscles degeneration and latter the regeneration of the damaged muscles was established , also histopathological changes on ( 2h , 2d , 5d , 10d , 15d ) post injection was clarified in Triceps brachii and Gastronemius muscles , in addition biochemical parameters which associated with muscles toxicity was evaluated. Total number ( 90 ) rats male (Rattus norvegicus ) was used in this study as experimental animals , all animals with weight ( 250 – 300 ) gm and ( 12 -14 ) weeks old . The rats divided into two group , first group regarded as control which injected intramuscularly with ( 0.6) ml of normal saline into each triceps and gastronemius muscle,second group injected with (0.6) ml of ( 0.5% Bpvc ) mixed with same volume of normal saline to induced myotoxicity . Rats were anaesthetic and muscles were excised from each rat at ( 2h ,2d , 5d, 10d , 15d ) post injection , these muscles prepared for light microscopy exam, Blood Samples were collected and serum separated ,stored for determined the biochemical tests ( Protein content , Calcium concentration , Alkaline phosphatase level ) in both control and treated rats. Histopathological changes after ( 2h ) in muscles injected with ( Bpvc ) included degeneration ,necrosis ,hypercontracted flber ,the damage extend to the deeper layer while normal nerves and blood vessel's were noticed . After ( 2d ) post injection the histological exam showed myonecrosis , dense inflammatory cells most of macrophages and polymorphonuclear cells ( PMN) , the muscle | ||
| Keywords | ||
| Study on Skeletal muscles during myotoxicity induced by | ||
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