Assessment of in vitro fertilization and early embryonic development using SMART medium enriched with coenzyme Ql0 | ||
| Iraqi Journal of Embryos and Infertility Researches | ||
| Article 1, Volume 4, Issue 1, June 2014, Pages 37-42 | ||
| Authors | ||
| Muhammad-Baqir M-R .Fakhrildin; ; A. AL-Bakri; Mudhafar A-Hussen Muhammad | ||
| Abstract | ||
| Background Zygote produce from once a sperm fertilizes an egg cell. Then, the zygote (unicellular) will begin chain of cellular cleavages to produce multicellular mass, its embryo, the differentiated to different tissues and organism.The development of the embryo is called embryogenesis. Coenzyme Q10, is an antioxidant produced in the body. It boosts cellular energy and may enhance the immune system. CoQ10 is present and measurable in seminal fluid,the concentration of CoQ10 directly correlates with both sperm count and motility. It is beneficial inthe prevention and treatment a wide range of health problems. Objectives The present study was aimed to investigate the possibility of using coenzyme Q1O to improvein vitro fertilization (IVF), and early embryonic development (ED) in mice as a model for human being Methods Superovulation program was achieved to mature healthy female mice with age 10-12 weeks and weight 24-26 gm. After sacrificing female, oocytes were collected and incubated within C02 incubator for less than 1 hour. Sperm were collected from vas deference of males. Sperm parameters were assessed after 30 min. of incubation. Mature oocytes were divided intothree groups accordingto theconcentrations of CoQ10 including G1 (control group; SMART medium only), G2 (treated group; SMART medium enriched with 20 M CoQ10) and G3 (treated group; SMART medium enriched with 40 M CoQ10). IVF technique was performed for 3 groups, and assessment of IVF (%), embryonic development stage (%) and abnormal embryo morphology (%) for each embryo stage. Results Results of the present study appeared significant increment (P<0.05) in the percentages of IVFfor both treated groups as compared to the control groups.Also, significant increase (P0.05%) in the 8-cells embryo stage were assessed among control and treated groups Conclusion From the results of the present study it wasconcluded that the coenzyme Q1O (40µM) enriched to the culture medium improved percentage of in vitro fertilization and no effect on embryonic development. | ||
| Keywords | ||
| fertilization; Embryo; smart; coenzyme Q | ||
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