Separation and Study Isoenzymes of Soluble Acetyl Cholineesterase in Normal Human Brain and Gliomas Tumors | ||
| journal of al-qadisiyah for pure science(quarterly) | ||
| Article 1, Volume 12, Issue 1, March 2012, Pages 112-120 PDF (0 K) | ||
| Authors | ||
| Yasera K.J; Fatin F. AL-Kazzaz; Raad K. M | ||
| Abstract | ||
| Soluble AchE had extracted from human brain (control)and Gliomas with a percent(21.08)of the total enzyme activity.Ammonium sulphate fractionation of solublel AchE extract from the human brain precipitated at (30-50)%saturattion with fold purification of (2.17).The enzyme was partially purified by Gel filtration chromatography on Sepharose-6Bgel where two peaks was obtained (named A,B) with fold purification(19.00and(24.1)respectively.Polyacrylamide gel(7.5%) electrophoresis revealed three bands of enzymatic activity for partially purified soluble AchE(control human brain),while two bands were separated from crude soluble AchE (Gliomas).The optimum reaction condtions: [s],pH and temperature had been estimated for both individual isoenzymes exhibited significantdifferece in some kinetic prprties including Vmax ,Kmand pH optima, while optimum substrate and temperature were examined without finding any significant difference between the formsbefore and after purification. | ||
|
Statistics Article View: 52 PDF Download: 40 |
||