Antimicrobial Effect of Bacteriocin produced Pediococcus pentosaceus on some clinical isolates | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 27, Issue 5, December 2016, Pages 26-30 | ||
| Author | ||
| Nehad A. Taher | ||
| Abstract | ||
| About 10 isolates of Pediococcus sp were isolated from different cheese made in Iraq, These isolates were identified morphologically and biochemically and Api20 kit, thus there was only 6 isolate were identified as Pediococcus pentosaceus (60%).In this study, we investigate, the effect of crude Bacteriocin from Pediococcus pentosaceus on 30 clinical isolates (5 E.coli, 5 Klepsiella pneumoniae, 5 Staphylococcus aureus, 5 Pseudomonas aeroginosa, 5 Bacillus subtilis, 5 Candida albicans). The protein concentration of this Bacteriocin was measured 67mgml by Bradford method and used as (1:2) by vol during the measuring the antimicrobial activity against the above clinical isolates by two methods wells and agar plug assay. The results showed that the inhibitory activity of this Bacteriocin was higher by wells method than agar pluq assay against Gram–positive bacteria or Gram-negative bacteria and yeast under this study. | ||
| Keywords | ||
| Antimicrobial; Bacteriocin; Pediococcus pentosaceus | ||
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