Molecular Study of P53- and Rb-Tumor Suppressor Genes in Human Papilloma Virus-Infected Breast Cancers | ||
| Journal of University of Babylon | ||
| Article 1, Volume 25, Issue 3, June 2017, Pages 901-914 | ||
| Authors | ||
| Shakir H. Mohammed Al-Alwany; Saad Hasan M. Ali; Ibrahim Mohammed S. Shnawa | ||
| Abstract | ||
| The study was aimd to define the percentage of detection of high- oncogenic risk types of HPV and their genotyping in archival tissue specimens that ranged from apparently health tissue to invasive breast cancer by using one of the recent versions of In Situ hybridization(ISH) .2.To find out rational significance of such genotypes as well as over expressed products of mutants P53 and RB genes on the severity of underlying breast cancers . The DNA of HPV was detected in 46.5 % of tissues from breast cancers while HPV DNA in the tissues from benign breast tumors was detected in 12.5%. No HPV positive – ISH reaction was detected in healthy breast tissues of the control group. HPV DNA of genotypes (16, 18, 31 and 33) were detected in malignant group in frequency of 25.6%, 27.1%, 30.2% and 12.4%, respectively. Over expression of p53 was detected by IHC in 51.2% breast cancer cases and in 50% benign breast tumor group , while none of control group showed P53- over expression . Retinoblastoma protein was detected by IHC test in 49.7% of malignant breast tumors, 54.2% of benign breast tumors but no signal was reported in the tissues of control group . | ||
| Keywords | ||
| HPV; breast tumors; ISH; IHC | ||
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