Immunological study among Tuberculosis patients in Baghdad | ||
| IRAQI JOURNALOF COMMUNITY MEDICINE | ||
| Article 1, Volume 28, Issue 4, December 2015, Pages 202-206 | ||
| Authors | ||
| May. Y. Abdul-Hussein; Oruba Kh. Abbas; Ahmed A. Mankh | ||
| Abstract | ||
| Background: Mycobacterium tuberculosis (Mtb) infection may cause overt disease or remain latent. Interferon gamma release assays (IGRAs) detect Mtb infection, both latent infection and infection manifesting as overt disease, by measuring whole blood interferon gamma (IFN-Ɣ) responses to Mtb antigens such as ESAT-6, CFP-10 and TB7.7. Biomarkers are crucial to the development of new diagnostic tools for tuberculosis diagnosis and could be instrumental in reducing morbidity and mortality and curtailing spread of tuberculosis. In this study, cytokines such as Tumor necrosis factor –alpha (TNF-α) and Interleukine-10 (IL-10) in addition to IFN- Ɣ were assessed as biomarkers of tuberculosis infection and reactivation; tuberculosis disease and tuberculosis cure. Objective: This study was designed to evaluate the immune status of patients with respect to Mycobacterium tuberculosis. A prospective study was conducted to obtain more clarification about the impact of causative agent . The study included immunological diagnosis by Quantiferon –TB Gold-Test (QFT) and estimation the serum levels of IFN-ɣ, TNF-α and IL-10 in TB patients and control. Methods: The present study included fifty TB patients attending the National Reference Laboratory of Tuberculosis /Baghdad during the period April to July 2014 were recruited for this study. For the purpose of comparison, 50 control samples (respiratory disease and healthy ) matched by age and gender were also included. The patients and control groups were examined for the presence of TB by Quantiferon TB Gold test. Results: Forty five samples of TB patients with significance difference (p≤0.01) were positively detected by Quantiferon-TB Gold Test. The present study revealed significant difference in IFN-ɣ, TNF-α and IL-10 levels between TB patients and control groups. Conclusion: High levels of Interferon-gamma, Tumor necrosis factor-alpha and Interleukine-10 in tuberculosis patients serum indicate an important role of this cytokines in the pathogenesis of tuberculosis, so they could be considered as a good biomarkers for diagnosis and considered as a target for future therapy. | ||
| Keywords | ||
| Quantiferon; tuberculosis; Tumor necrosis factor; Alpha; Interleukine; IFN; TNF | ||
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