abd Al-salam Obeed, D., Qais Abd, M., Mohamed Abd, E. (2015). Using different method for detection Helicobacter pylori that causing gastric cancer and compared with invasive method in the Al-Ramadi city. Alustath, 9(3), 107-113. doi: 10.37652/juaps.2015.127617
Dhuha abd Al-salam Obeed; Mohammed Qais Abd; Essam Mohamed Abd. "Using different method for detection Helicobacter pylori that causing gastric cancer and compared with invasive method in the Al-Ramadi city". Alustath, 9, 3, 2015, 107-113. doi: 10.37652/juaps.2015.127617
abd Al-salam Obeed, D., Qais Abd, M., Mohamed Abd, E. (2015). 'Using different method for detection Helicobacter pylori that causing gastric cancer and compared with invasive method in the Al-Ramadi city', Alustath, 9(3), pp. 107-113. doi: 10.37652/juaps.2015.127617
abd Al-salam Obeed, D., Qais Abd, M., Mohamed Abd, E. Using different method for detection Helicobacter pylori that causing gastric cancer and compared with invasive method in the Al-Ramadi city. Alustath, 2015; 9(3): 107-113. doi: 10.37652/juaps.2015.127617

Using different method for detection Helicobacter pylori that causing gastric cancer and compared with invasive method in the Al-Ramadi city

Journal of University of Anbar for Pure Science
Article 20, Volume 9, Issue 3, December 2015, Pages 107-113    PDF (689.19 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2015.127617
Authors
Dhuha abd Al-salam Obeed* 1; Mohammed Qais Abd1; Essam Mohamed Abd2
1University of Anbar - College of Science
2University of Anbar - College of Pharmacy
Abstract
The study aims at finding out the most efficient method in the diagnosis and the easiest sample in usage. The study collects 50 samples from outpatients who customarily visit the medical sight unit. The collections of the samples include biopsy tissue, blood, saliva and stool from each patient, as well as 30 samples were collected from the control group (people who are not infected). The samples of biopsy tissue are used to test (Culture, rapid urease enzyme, and histopathology) through using saliva sample of the culture test. It also uses stool samples, rapid antigen stool test (strip) . However, the serum samples were used to detect antibodies formed IgG against infection with the Helicobacter pylori . The study compared between the invasive and non- invasive. the culture test was 6% of sensitivity and 100% of specificity, the rapid urease enzyme test was 66% of sensitivity and 90% of specificity and the histopathologic test was 14% of sensitivity and 100% of specificity. The rapid stool antigen test was 54% of sensitivity and 80% of specificity then the serum ELISA test with a sensitivity of 58% and specificity of 60%. It was found after comparing invasive with non-invasive methods that the rapid urease enzyme in invasive methods is the most efficient in diagnosis , also The non-invasive methods found the rapid stool antigen test the most efficient in diagnosis .
Keywords
Helicobacter pylori
References

 

  1. Atherton, J. , (2006). The pathogenesis of Helicobacrter pylori induced gastroduodenal diseases. Journal Annual Review of Pathology: Mechanisms of Disease., Vol. 1: pp.63.
  2. Mahady, G.B.; Pendland, S.L. ; Stoia, A.; Hamill, F.A.; Fabricant, D.; Dietz, B.M. ; and Chadwick,.LR., (2005). In vitro susceptibility of Helicobacter pylori to botanical extracts used traditionally for the treatment of gastrointestinal disorders. Journal Phytother. Res.,Vol. 19:pp. 988-991.
  3. Goodwin, S.; Blincow, D.; Warren, R.; Waters, E.; Sanderson, R.;  and Easton, L., (1985). Evaluation of cultural techniques for isolating Campylobacter pyloridis from endoscopic biopsies of gastric mucosa. Journal Clin. Pathol. , Vol. 38: pp. 1127-1131.
  4. Garza-Gonzalez, Elvira ; Perez-Perez, Guillermo Ignacio ; Maldonado-Garza , Héctor Jesús ; and Bosques-Padilla , Francisco Javier , (2014).  A review of Helicobacter pylori diagnosis, treatment, and methods to detect eradication. journal World J Gastroenterol , Vol.  20 , No. 6 :  pp. 1438-1449.
  5. Atheron , John C.,(1998).H.pylori virulence factors. Journal , British medical Bulletin ; NO.1 : pp 105 - 120.
  6. Cover, T.L. ;  and Blaser, M.J., (2009). Helicobacter pylori in health and  disease.  Journal Gastroenterology.,Vol. 136: pp. 1863-1873.
  7. Luigina, Cellini1; Rossella ,Grande; Luciano , Artese; and Leonardo, Marzio.,(2010). Detection of Helicobacter pylori in saliva and esophagus. 33, 351-357.
  8. Kalaf ,  Elham Abdulhadi , (2013). Molecular Study for Detection of CagA Genotype of Helicobacter pylori from Endoscopic Biopsies of Iraqi Patients. University of Baghdad.
  9. Ali  , Sama Fakhri , (2013). Detection of Helicobacter pylori in saliva from some Iraqi patients in comparison with other methods. University of Baghdad.
  10. Ashok, Kumar ; and Imran , Khan.,(2010). Detection of Helicobacter pylori in Gastroduodenal Diseases by Real Time PCR.  170-178.
  11. Zsikla, V. ; Hailemariam, S.  ; and Baumann, M., (2006) Increased rate of Helicobacter pylori infection detected by PCR in biopsies with chronic gastritis. Journal The Am.J. of Surgical Pathology., Vol. 30 , No.  2 : pp.  242–248.
  12. Arkckila ,P.E. ; Seppala , K. ; Kosunen , T.U. ; Sipponen, P. ; Makinen, J. ; Rautelin , H. ; and Farkkila, M. , (2005). Helicobacter pylori eradication as the sole treatment for gastric and duodenal ulcers. Eur. Journal Gastroenterol. Hepatol , Vol. 17: pp.93-101.
  13. Wong, B.C.;  Lam,  S.K. ; Wong, W.M. ; Chen, J.S.; Zheng, T.T.; Feng, R.E. ; Lai, K.C.; Hu, W.H.; Yuen , S.T.; Leung ,S.Y. ; Fong, D.Y.; Ho, J. ; Ching , C.K. ; and Chen, J.S., (2004). Gastric Cancer Study Group: Helicobacter pylori eradication to prevent gastric cancer in a high-risk region of China: a randomized controlled trial. Journal JAMA , Vol. 291: pp.187-94.
  14. Wong , B.C.; Zhang , L.; Ma, J.L. ; Pan, K.F. ; Li , J.Y.; Shen , L. ; Liu , W.D. ; Feng , G.S. ; Zhang, X.D. ; Li, J. ;  Lu , A.P. ; Xia , H.H. ;  Lam, S. ; and You , W.C. , (2012). Effects of selective COX-2 inhibitor and Helicobacter pylori eradication on precancerous gastric lesions. Journal Gut , Vol. 61: pp. 812-818.
  15. Brown, L.M. , (2000). Helicobacter pylori: Epidemiology and routes of transmission. Journal Epidemiol. Rev., Vol 22: pp.238-297.
  16. Fukuda , K. ; Kuroki , T. ; Tajima , Y. ; Tsuneoka m N. ; Kitajima , T. ; Matsuzaki , S. ; Furui , J. ; and Kanematsu , T. , (2002). Comparative analysis of Helicobacter DNAs and biliary pathology in patients with and without hepatobiliary cancer. Journal Carcinogenesis , Vol. 23 , pp. 1927 – 1931.
  17. Bakir,  Wasan A. , (2007). Immunological and Molecular Studies on Gastroduodenal Diseases Caused by Helicobacter pylori. University Anbar.
  18.  Suerbaum ,S. ; and Michetti , P. , (2002). Helicobacter pylori infection. Jornal N Engl J Med, vol. 347: pp. 1175–1186.
  19. Sowjanya , Kanna ; Carla , Maradey-Romero;  and  Ronnie, Fass ,(2013). Diagnostic tests for Helicobacter pylori.Journal Castroenterology  Endoscopy News .
  20. Hong, LU ; and Shu,  Dong XIAO , (2014). New ideas for future studies of Helicobacter pylori. Journal of Digestive Diseases, Vol. 15: pp. 1-4.
  21. Pereira, Weendelly Nayara; Ferraz, Mariane Avante; Zabaglia, Luanna Munhoz; de Labio, Roger William; Orcini, Wilson Aparecido; Ximenez , Joao Paulo Bianchi ;  Neto , Agostinho Caleman ; Payao ,Spencer Luiz Marques; and Rasmussen, Lucas Trevizani , (2014). Association among H. pylori virulence markers dupA, cagA and vacA in Brazilian patients Journal of Venomous Animals and Toxins including Tropical Diseases,Vol.20 : pp.1.
  22. AL-rubai , Aseel Ibrahim , (2006). The role of lipopolysaccharide in pathogenesis of Helicobacter pylori isolated from patients suffering from duodenal ulcer.B.Sc.,  Almustunsirya University.
  23. Boyanova, L. ,(2011). Helicobacter pylori. Journal Caister Academic press.
  24.  Simona , Cardaropoli ; Alessandro ,  Rolfo ; and Todros Tullia , (2014). Helicobacter pylori and pregnancy-related disorders. Journal  World J Gastroenterol ; Vol. 20, No. 3: pp 654-664.
  25. Luo, Jiing-Chyuan , (2015). Noninvasive diagnostic methods for Helicobacter pylori infection. Journal of the Chinese Medical Association , Vol. 78 : pp. 83-84.
  26. Khalifehgholi, Mohammad ; Shamsipour, Fereshteh ; Ajhdarkosh, Hossein ; Daryani, Naser Ebrahimi ; Pourmand ,Mohammad Reza; Hosseini , Mostafa ; Ghasemi  , Amir ; and Shirazi, Mohammad Hasan , (2013). Comparison of five diagnostic methods for Helicobacter pylori. Journal IRAN. J. MICROBIOL. Vol. 5, No. 4 : pp. 396-401.
  27. Patel , Saurabh Kumar ; Pratap,  Chandra Bhan; Jain, Ashok Kumar ; Gulati ,Anil Kumar; and Nath, Gopal , (2014). Diagnosis of Helicobacter pylori : What should be the gold standard ?. Journal  World J Gastroenterol , Vol. 20 No.36 : pp. 12847-12859. DOI: 10.3748/wjg.v20.i36.12847.
  28. Andrews, J. ; Marsden, B. ; Brown, D. ; Wongm V S. ; Woodm E. ; and  Kelsey, M. ,( 2003). Comparison of three stool antigen tests for Helicobacter pylori detection. Journal J Clin Pathol , Vol. 56: pp. 769–771.
  29. Reddy, B. Shanti ; Venkateswarlu, P. ; Jyothi ,B. Naga ; and Devi, A. Renuka , (2015).  Role of  H. pylori in Gastroduodenal  Diseases. Journal of Evolution of Medical and Dental Sciences , Vol. 4, Issue 04,  :pp.  581-586. DOI: 10.14260/ jemds /2015/86.
  30. Uotani , Takahiro; and Graham, David Y. , (2014). Diagnosis of Helicobacter pylori using the rapid urease test. Journal Annals of Translational Medicine , Vol. 3 No. 1 : pp. 9. doi: 10.3978/j.issn.2305-5839.2014.12.04.
  31. Megraud , F.;  Bessede , E. ; and Lehours, P., (2014). Current methods used for the diagnosis of  Helicobacter pylori infection. In: Buzas GM. eds. Helicobacter pylori - A Worldwide Perspective. Journal Oak Park: Bentham Science, Vol. 2014 : pp.234-58
  32. Farah, Hamza.J.M.; Adam, Elsadig A.; El-fatih , Mohamed; Abdalla , Mohammed. A.; Ibrahim, Fathelrahman.M.A.;and Ibnouf, Abd-Alhafeez , (2015).  Detection of H. Pylori by Different Conventional Staining Methods and Immunohistochemistry in Sudanese Patients with Chronic Gastritis. Journal American Journal of Research Communication ,Vol. 3 , No. 2.
  33. Lee  , Ju Yup ; and Kim, Nayoung , (2014). Diagnosis of Helicobacter pylori by invasive test: histology. Journal Annals of Translational Medicine , Vol. 3 No. 1 : pp. 10. doi: 10.3978/j.issn.2305-5839.2014.11.03.
  34. Siavoshi, Farideh ; Saniee ,Parastoo ; Khalili- Samani ,Saman ; Hosseini, Farideh ; Malakutikhah , Fahimeh ; Mamivand , Marzieh ; Shahreza , Somayeh ; and Sharifi , Amir Houshang , (2014). Evaluation of methods for H. pylori detection in PPI consumption using culture, rapid urease test and smear examination. Journal Annals of Translational Medicine  , Vol. 3 No. 1 : pp. 11. doi: 10.3978/j.issn.2305-5839.2014.11.16.
  35. Song, Q. ; Lange, T. ;and Sphar, A. , (2000). Characteristic distribution pattern of Helicobacter pylori in dental plaque and saliva detected with nested PCR. Journal Med. Microbiol, Vol. 49: pp. 349–353.
  36. Olivier, B. J. ; Bond, R. P. ; van Zyl, W. B. ; Delport, M. ; Slavik, T. ; Ziady, C. ; Terhaar, Sive. Droste, J. S. ; Lastovica, A.; and van der Merwe, S. W., (2006). Absence of Helicobacter pylori within the oral cavities of members of a healthy South African community. Journal Clin. Microbiol, Vol. 44: pp. 635–636.
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