Effect of Methotrexate and Omega 3 in Some Parameters Related With WBCs and RBCs in Male Rats | ||
| Journal of University of Anbar for Pure Science | ||
| Article 1, Volume 9, Issue 3, December 2015, Pages 1-5 PDF (490.3 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2015.127543 | ||
| Authors | ||
| Muthana M. Awad* 1; Inaam N. Ali2; Alaa Sh. Mahmood3 | ||
| 1University of Anbar -College of Sciences | ||
| 2Ministry of Sciences and Technology- Water and Environment Directory | ||
| 3University of Anbar - College of Science | ||
| Abstract | ||
| The current study included understanding the effect of methotrexate and omega-3 in the thyroid and parathyroid gland and some of the physiological and histological and genetic variables in some of the organs of the body in the male rats. The study included 56 animals were divided into three groups, the first and second groups were divided into three secondary groups, the first secondary group was given the drug methotrexate only in concentration 0.05 , 0.125 and 0.250 mg / kg for 8 weeks while the secondary second group was given drug methotrexate in the same concentrations of the first group, as well as give it omega-3 concentration of 300 mg / kg for 8 weeks, the third group was considered as control group. After finishing of dosage, the animals killed and took blood samples and organs to do the required tests, included measuring of blood parameters, such as RBC ,PCV ,Hb ,MCV, MCH and MCHC. In addition to measure the number of white blood cell count WBC and Differential white blood cells, where the results showed the presence of a rise in both the RBC, PCV, Hb in the low and medium concentration of methotrexate while decreased in high concentration, omega-3 work to reduce the variables above in the low and medium concentration while worked on reducing it in the high concentration, moreover a variable of MCV and MCH and MCHC has not changed morally. With respect to the WBC was low in the totals for the study when giving methotrexate while rose when giving omega-3 with this druge, also neutrophil cells was low and accompanied by a rise in a monoytes in all concentrations of Methotrexate were not for omega 3 noticeable effect on these cells decreased. The results showed a decline in the lymph and acidophilus cells in the low concentration while rose in the medium and high concentration of Methotrexate. Omega-3 worked on the rise it in concentrations above, with respect to basophiles cells increased in the low and high concentration and decreased in medium and the omega-3 was not effect of them. | ||
| Keywords | ||
| Methotrexate; Omega; RBCs; WBCs | ||
| References | ||
|
[1] Yuen, C.W. and Winter, M.E.( 2010) In Methotrexate (MTX) in Basic clinical pharmacokinetics; Michael E. Eds.; Winter-5th; Wolters Kluwer/Lippincott Williams & Wilkins: Philadelphia, USA; pp. 304–325.
[2] Vezmar S, Becker A, Bode U and Jaehde U.( 2003). Biochemical and clinical aspects of methotrexate neurotoxicity. Chemotherapy. 49: 92-104.
[3] Tian H and Cronstein B N.(2007). Understanding the Mechanisms of Action of Methotrexate Implications for the Treatment of Rheumatoid Arthritis. Bulletin of the NYU Hospital for Joint Diseases; 65(3):168-73.
[4] El-khayat, Z.; Rasheed, W.I.; Elias, T.R.; Hussein, J.; Oraby, F.; Badawi, M. ; Salem, S. (2010). Protective effect of either dietary or pharmaceutical n-3 fatty acids on bone loss in ovariectomized rats. Maced. J. Med. Sci., 15; 3(1), 9-16
[5] Richell,B.D. (2001). Fish oil, fish consumption, omega -3 fatty acids And cardiovascular disease. J. Nutr.160: 1050 -1055.
[6] Calder PC.( 2006). Polyunsaturated fatty acids and inflammation Prostaglandins Leukot Essent Fatty Acids. 75(3):197-202.
[7] Begin ME, Ells G, Das UN et al.( 1986). Differential killing of human carcinoma cells supplemented with n-3 and n-6 polyunsaturated fatty acids. J Natl Cancer Inst. 77:1053–1062.
[8] Shan, B., Cai, Y. Z., Sun, M and Corke, H. (2005). Antioxidant capacity of 26 spice extracts and characterization of their phenolic constituents. J. Agri. and Food Chem., 53:7749-7759.
[9] Matiax , J. ; Quiles, J.L. ; Huertas, J.R. and Battino, M. , Free Rad. BioL. Med. (1998);24 : 511- 521.
[10] Dean , R.T. ; stocker R. and Davies, M.J. , Mediated protein oxidation (1997); 324:1.
[11] Bashir, A.; Perham, R. N.; Scrutton,N. S. and Berry, A. (1995) Altering Kinetic mechanism and enzyme stability by mutagenesis of the dimmer interface of glutathione reductase. Biochem.J. 312 : 527-53.
[12] Perret-Gentil M I. (2010). Rat Biomethodology. University Veterinarian & Director. Laboratory Animal Resources Center.The University of Texas at San Antonio. (210) 458-6173.
[13] Henry, J. B. and Davidson, I. (1974) . Clinical diagnosis by laboratory methods. Saunders Company . London .
[14] Powers, L. W. , Diagnostic Hematology : Clinical and Technical Principles , Mosby Company. United States of America. 1989.
[15] Sood, R. ( 1985 ) . Hematology for studen and practitioners . India Jappe brothers , pp. 243 – 320 .
[16] Hillman R. and Ault K. (2002). Hematology in Clinical Practice. 3rd ed., McGraw- Hill. pp. 46-47.
[17] Shen, P.F. and L.T. Patterson. (1983). A simplified wrights stain technique for routine avian blood smear staining. Poultry sci., 62: 923-924.
[18] Burton, R.R. and C.W. Giuton. (1968). The differential Leucocytes blood count: Its precision and individuality in the chicken. Poultry Sci., 47: 1945-1949.
[19] SAS. (2012). Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA.
[20] Bachman E, Travison TG, Basaria S, Davda MN, Guo W, Li M, Connor Westfall J, Bae H, Gordeuk V, Bhasin S.(2014). Testosterone Induces Erythrocytosis via Increased Erythropoietin and Suppressed Hepcidin: Evidence for a New Erythropoietin/Hemoglobin Set Point. ournals of Gerontology: MEDICAL SCIENCES. 69(6):725–735.
[21] Roy, R.; Singh, S. and Pujari, S. (2008). Dietary role of omega-3 poly unsaturated fatty acid (PUFA): A study with growing chicks, Gallus domestic's. Int. J. Poult. Sci., 7(4): 360 – 367.
[22] Iranloye , B.O. (2002) . Effect of chronic garlic feeding on some haematological parameters . African J . of Biomed . Res . 81-82.
[23] AL-Haidari , S . and AL-Alwan , H . (2002) . Red cell distribution within pediatric microcytic amenia . Fac . Med . J . 44 (1) : 117-124.
[24] Schroeder, M. A. and Dipersio, J. F. (2012). Mobilization of hematopoietic stem and leukemia cells. J. of Leukoc. Biol. 91(1):47-57.
[25] Ohbayashi, M., Suzuki, M., Yashiro, Y., Fukuwaka, S., Yasuda, M., Kohyama, N., Kobayashi, Y. and Yamamoto, T. (2010) Induction of pulmonary fibrosis by methotrexate treatment in mice lung in vivo and in vitro. J Toxicol Sci. 35(5): 653-661.
[26] Biswas A., Ahmed M., Bharti V. K. and Singh S. B.(2011). Effect of Antioxidants on Physio-biochemical and Hematological Parameters in Broiler Chicken at High Altitude. Asian-Aust. J. Anim. Sci.24(2): 246 – 249.
[27] Guyton, C. and Hall, J. E. (2011). Textbook of Medical Physiology . 12th edition. ISBN 0-7216-0240-1. Elsevier . Inc. 865-878.
[28] Coskum, H.S.; Ozlem, ER.; TanriverdiF.andAltinbas M. (2003) . Hypereos- inophilia as a preclinical sign of tongue squamous cell cancer patient with complete remission . Turk J. Haematal . 20(2) : 107-110.
[29] Deuel J W , Lutz H U , Misselwitz B.(2012). Asymptomatic elevation of the hyperchromic red blood cell subpopulation is associated with decreased red cell deformability. Ann Hematol 91:1427–1434.
[30] Patel N. N., Ghodasara D. J., Pandey S., Ghodasara P.D., Khorajiya J. H., Joshi B. P. and Dave C. J.(2014). Subacute toxicopathological studies of methotrexate in Wistar rats. Veterinary World;7:489-495.
[31] Haung M J and Liaw Y F.(1995). Clinical associations between thyroid and liver diseases. Journal of Gastroenterology and Hepatology ;10,344-350.
| ||
|
Statistics Article View: 2,161 PDF Download: 446 |
||