Resistance to cecal coccidiosis following sonicated oocysts immunization of Eimeri tenella in broilers | ||
| Kufa Journal For Veterinary Medical Sciences | ||
| Article 1, Volume 6, Issue 1, June 2015, Pages 49-55 | ||
| Authors | ||
| Latif I. Kadhim; Awse. M. Hussien | ||
| Abstract | ||
| Seventy five broiler chicks were used to determine the immunization of in ovo inoculation or intramusclular (I/M) injection of sonicated oocyts (SO) antigen against E. tenella manifestation. The chicks were divided in to 3 equal groups compromising of 25 chicks each. The 1st group inoculated in ovo with SO of E. tenella and repeated by I/M injection at 21 days old. The 2nd group injected I/M with SO at day old and repeated at 21 days old, The birds of the 3rd group remains as control. All birds were challenge by 50,000 sporulated oocysts of E. tenella at day 28. No significant (P > 0.05) group difference was detected between the immunized and non-immunized groups for mean, WBC count, H/L ratio, lesion score, mortality and oocysts shedding until E. tenella challenge at day 28. After challenge test the in ovo and I/M immunized groups showed a great protective immunity against E. tenella infection documented by significantly (P < 0.05) reduced mortality, lesion score and decreased fecal oocyst shedding, compared with non-immunized group. It is concluded that in ovo and I/M immuinzation of sonicated oocyst stimulates a remarkable protection in broilers following E. tenella infection comparing with non-immunized groups. There was no significance difference between the two procedures of immunization. | ||
| Keywords | ||
| tenella; immunity; sonicated oocyts; Broilers | ||
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