Effect of Explant and Methyl Jasmonate on Prduction of Phenolic Compounds In vitro of Salvia officinalis | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 17, Issue 2, July 2017, Pages 76-86 | ||
| Author | ||
| Shamil I. Neamah | ||
| Abstract | ||
| This experiment was conducted at plant tissue culture Laboratories, College of Agriculture, University of Baghdad. The objectives of this study effect of explant and methyl jasmonate in the production of phenolic compounds in callus of buckwheat Salvia officinalis. The concentrations of methyl jasmonate and the second interaction caused by a significant reduction in average callus fresh weight in hypocotyl and cotyledon. The results showed that the concentration of methyl jasmonate significantly effected at the content of phenolic compounds at the callus inducted from hypocotyl and cotyledon. The best concentration of methyl jasmonate stimulated the production was 300 µM for phenolic compounds reached to 11.47, 254.12, 36.90, 26.59 and 92.80 µg.100mg-1 weight fresh for Vanillic acid, Rosmarinic acid, Quercetin, Naringinin and Apigenin. Also, gave same concentration a significant increase of Coumaric acid 19.60 µg.100mg-1 weight fresh when extract in callus inducted from hypocotyl. But, the concentration of methyl jasmonate 200 µM with the callus inducted from cotyledon gave the highest product for Chlorogenic acid and Luteolin 29.59 and 77.68 µg.100mg-1 weight fresh. And, Caffeic acid acid reached 25.97 µg.100mg-1 weight fresh at the concentration 100 µM of methyl jasmonate at callus inducted from cotyledon. | ||
| Keywords | ||
| Salvia officinalis; phenolic compounds; Methyl Jasmonate | ||
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