Immune and non-immune diagnosis of H. pylori In Patients with ingastric ulcer (GU) and non-ulcer dyspepsia (NUD). | ||
| Journal of University of Anbar for Pure Science | ||
| Article 4, Volume 8, Issue 3, December 2014, Pages 15-19 | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2014.123988 | ||
| Authors | ||
| Aseel k. Husain1; Shehab A. Lafi2; Yasin H. Al-Fahdawi1 | ||
| 1Univers ity of Anbar - College of Medicine | ||
| 2Univers ity of Anbar - College of Medicine. | ||
| Abstract | ||
| Background:The roles that T helper type 1 (Th1) specific immune responses in protection from H. pylori challenge was understood. It is expected that Th2 immune responses are required for protection against extracellular bacteria, such as H. pylori. Both invasive and non-invasive tests are used in the diagnosis of Helicobacter pylori infection. The non-invasive tests avoid endoscopy and encompass the serologic and breath tests This study aimed to show important immune and non-immune tests for Helicobacter pyloriinfection diagnosis in patients . Patients and Methods:A total of one hundred seven (107) adult patients from both genders were attending Gastro Endoscopy Unit at Ramadi Teaching Hospital to undergo selective OGD from December 2012 to May 2013. Multiple mucosal biopsy specimens were taken for rapid urease test (RUT) to detect Helicobacter pylori in tissue samples. After endoscopy, blood specimen was taken from each patient to be used for serological tests including; IgG, IgM, by ELISA. Rapid Chromatographic Immunoassay test (CAS) was used for IgG against H.pylori also . Results: Present study showed that the rate of infection in males was same as in females, and increased within age group (31-50) years old, it was found thathigher positive results of CAS, and RUT for H. pylori, especially in younger adults. Findings confirmed that a significant relationship between H. pylori rapid urease test (RUT) with IgG and IgM specific for H. pylori antigen. | ||
| Keywords | ||
| immune; non; immune diagnosis; pylori; ingastric ulcer; ulcer dyspepsia | ||
| References | ||
|
1- Sainz R., Borda F., Dominguez E., and Gis bert JP: Helicobacter pylori infection. The Spanis h cons ens us report. The Spanis h cons ens us conference group. Rev Es p Enferm Dig. 1999; 91:777-784.
2- Muller L.B., Fagundes R.B., Moraes C.C. and Rampazzo A.: Prevalence of Helicobacter pylori infection and gas tric cancer precurs or les ions in patients with dyspepsia. Arg. Gastroenterol. 2007; 44:93-98.
3- Is rael D. A., and Peek R. M.: Pathogenes is of Helicobacter pylori-induced gas tric inflammation. Aliment. Pharmacol. Ther. 2001;15: 1271-129.
4- Kuwahara H., Miyamoto Y. &Akaike T. et al.: Helicobacter pylori Ureas e Suppres s es Bactericidal Activity of Peroxynitrite via Carbon Dioxide Production . Infectection and Immunity. 2000. 68;(8): 4378–4383.
5- Pars onnet J, Hans en S, Rodriguez L, et al.: Helicobacter pylori infection and gas tric lymphoma. N Engl J Med. 1994; 330:1267- 1271.
6- Alevizos L., Gomatos , I.P. Smparounis S., et al.: Review of the molecular profile and modern prognos tic markers for gas tric lymphoma: How do they affect clinical practice?. Can. J. Surg. 2012; 55 (2): 117-124.
7- Me´graud F. and Lehours P.: Helicobacter pylori Detection and Antimicrobial Sus ceptibility Tes ting. Clinical Microbiology Reviews. 2007; 20(2): 280–322.
8- Goh K., Chan W., Shiota S. and Yamaoka Y.: Epidemiology of Helicobacter pylori Infection and Public Health Implications . Helicobacter. 2011; 16 (1): 1-9.
9- Tes terman T. L. and Morris J.: Beyond the s tomach: An updated view of Helicobacter pylori pathogenes is , diagnos is , and treatment. World J Gas troenterol. 2014; 20(36): 12781-12808.
10- Berry V. and Sagar V.: Rapid Ureas e Tes t to Diagnos e Helicobacter Pylori Infection. J K Science. 2006; 8(2): 86-88.
11- Rahbar M., Mardanpur K., and Tavafzadeh R.: Imprint cytology: a s imple, cos t effectivenes s analys is for diagnos ing Helicobacter pylori, in wes t of Iran. Medical Journal of Is lamic Republic of Iran, 2012; 26(1),12.
12- Leong R.W., Lee C.C., Ling T.K., et al.: Evaluation of the s tring tes t for the detection of Helicobacter pylori. World J Gas troenterol. 2003; 9(2):309-11.
13- Vilaichone R.K., Mahachai V., Tumwas orn S. and Kullavanijaya P.: Gas tric ju ice ureas e tes t and brus hing ureas e tes t for Helicobacter pylori detection. J Med As s oc Thai. 2002; 85 (Suppl 1):S74-8.
14- Lopes A.I., Vale F.F, and Oleas tro M.: Helicobacter pylori infection - recent developments in diagnos is . World J Gas troenterol. 2014; 20(28): 9299-9313.
15- Gatta L., Vakil N., Ricci C., et al.: Effect of proton pump inhibitors and antacid therapy on 13C urea breath tes ts and s tool tes t for Helicobacter pylori infection. Am J Gas troenterol. 2004; 99(5):823-829.
16- Feldman R.A., Deeks J.J., and Evans S.J.: Multi-laboratory comparis on of eight commercially available Helicobacter pylori s erology kits . Helicobacter p ylori Serology Study Group. Eur J Clin Microbiol Enfect Dis . 1995; 14:428-433.
17- Gis bert J. P. and Pajares J. M.: 13C-urea breath tes t in the diagnos is of Helicobacter pylori infection. Aliment Pharmacol Ther. 2004; 20: 1001–1017.
18- Braden B., Teuber G., Dietrich C.F., et al.: Comparis on of new faecal antigen tes t with (13)C-urea breath tes t for detecting Helicobacter pylori infection and monitoring eradication treatment: pros pective clinical evaluation. BMJ. 2000; 320 (7228): 148.
19- Fallone C.A., Elizov M., Cleland P., et al.: Detection of Helicobacter pylori infectio n by s aliva IgG tes ting. Am JGastroenterol. 1996; 91(6):1145-9.
20- Kato M., As aka M., Saito M., et al: Clinicalus efulnes s of urine-bas ed enzyme-linked immunos orbent as s ay for detection of antibody to Helicobacter pylori: a collaborative s tudy in nine medical ins titutions in Japan. Helicobacter. 2000; 5(2):109-19.
21- Akhiani A.A., Stens s on A., Schon K., and Lycke N.Y.: IgA antibodies impair res is tance agains t Helicobacter pylori infection: s tudies on immune evas ion in IL- 10-deficient mice. J Immunol. 2005; 174:8144-8153.
22- Aydin F., Kaklikkaya N., Ozgur O., et al: Dis tribution of vacA alleles and cagA s tatus of Helicobacter pylori in peptic ulcer dis eas e and non -ulcer dys peps ia. Clin Microbiol Infect. 2004; 10: 1102 - 1104.
23- Me´graud F. and Lehours P.: Helicobacter pylori Detection and Antimicrobial Sus ceptibility Tes ting. Clinical Microbiology Reviews. 2007; 20(2): 280– 322.
24- Howden C.W., Hunt R.H.: Guidelines for the management of Helicobacter pylori infection. Ad Hoc Committee on Practice Parameters of the American College of Gas troenterology. Am J Gastroenterol.1998; 93(12): 2330-8. | ||
|
Statistics Article View: 2,268 PDF Download: 53 |
||