*Identify genetic variation for parasite strains of Echinococcus granulosus in humans and some intermedete host by using the polymerase chain reaction (PCR) | ||
| journal of al-qadisiyah for pure science(quarterly) | ||
| Article 1, Volume 16, Issue 2, June 2016, Pages 66-78 PDF (0 K) | ||
| Author | ||
| Hadi mdlol hamza | ||
| Abstract | ||
| Aneighteen of human hydatidosis sample were collected (18) the period of study from November 2012 until July 2013during a surgical procedure for patients with study wasrecored5.50%, examined previous samples using the technique of the amplified (RAPD) Random amplified polymorphic DNA to determine the genetic variation between strins using random primers are 6 (OPE - 07 OPF - 13 and OPF - 19andOPC-10 and OPF _16 and OPA –01) detecting genetic variation between samples through the presence or absence of serial packages DNA in the samples studied have shown the following prefixes -The ability of the primer (OPE - 07) to determine the DNA samples for the DNA of the hydatid dieases Aladrah excised from human liver when molecular weight 400bp and 650bp as an indicator of genetically excised samples of human liver did not appear in other samples Alamadaúv. -The ability of the primer (OPF - 13) to determine the DNA nucleic acid DNA in bags Aladrah to human liver when molecular weight 300bp and 500bp appeared in samples where the man did not appear in other samples Alamadaúv-The ability of the primer (OPC - 10) to determine the DNA nucleic acid DNA of bags Aladrah to human liver when molecular weight 500bp, 600bp, 700bp, 900bp, where the samples did not appear to Amadaúv other -Non ability of primer (OPF - 19, OPA - 01, OPF - 16) to determine any genetic fingerprint template is similar to DNA in human samples -Ability the primer (OPE - 07) to determine the DNA nucleic acid DNA in sheep when the molecular weight 200bp, 300bp, 500bp In Alabakarand molecular Hydatid dieases , fingerprints,weight 500bp was unable to identify any imprint hereditary in beauty and this is this signatures are fingerprints especially this Alamadaúv only possible which set them apart from the other samples studied. -Ability primer (OPF - 13) to determine the DNA nucleic acid DNA and excised from the livers of sheep when the molecular weight 300bp, 200bp, 400bp, 750bp and in cows 200bp,, 600bp, 700bp and was unable to identify any imprint hereditary in the DNA samples of the bags Aladrah excised of beauty. -Ability primer (OPF - 19) to determine the DNA nucleic acid DNA of bags Aladrah excised from the liver of sheep at 500bp at the molecular weight 300bp and 500bp and beauty at 1300bp. -Ability the primer (OPC - 10) to determine the DNA in the beauty at 1300bp and was unable to identify any genetic imprint in cattle and sheep. -Ability the primer (OPF_ 16) to determine the DNA nucleic acid of the bags Aladrah in sheep when the molecular weight 300bp, 400bp, 500bp and 300bp when cows and camels at 500bp 600bp. Finally, the study showed that the strains that affect humans have come from sheep and cows exclusively to the presence of fingerprints and genetic are shared between them can be detected using the initiators of OPE - 07 at the molecular weight 500bp and OPF - 13 at the molecular weight 300bp only, but this needs to be a larger study using the relay genetic Gen seqenses and all other pets near human. | ||
| Keywords | ||
| Key Words; polymorphic DNA Random amplifie | ||
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