Using the smear method and Real-Time PCR technique in diagnosis of Toxoplasma gondii parasite in white male rats experimentally infected by parasite | ||
| journal of al-qadisiyah for pure science(quarterly) | ||
| Article 1, Volume 16, Issue 4, December 2016, Pages 58-72 PDF (0 K) | ||
| Author | ||
| Ghaidaa Abbas Jasim | ||
| Abstract | ||
| The present study aims to isolate Toxoplasma gondii parasite and causing the experimentally infection and diagnosis of this infection by using the smear method and Real-Time PCR technique in different organs of white male rats such as brain, liver and testes . from aborted placentae of infected women and experimentally infection of male rats then diagnosed of this infection by impression smear that produced . The present study included 30 white male rats Rattus norvegicus aged ( 60-70) days averaged weight (200-250)gm . tissue cysts of parasite were presence in all prepared smears of brain ,liver and testes organs in different size and numerous numbers ,the percentage of infection by using Real-Time PCR was 26% in brain and 20% in liver and testes .this study was recorded the possibility of isolation of Toxoplasma gondii parasite and and diagnosis by using impression smears method and Real- Time PCR technique in different tissues of the organs of the experimental animals. | ||
| Keywords | ||
| Toxoplasma gondii; placentae; smears; tissue cysts; Toxoplasmosis; Real; time PCR | ||
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