Study the efficiency of aqueous extracts of green tea and licorice on sperm parameters and reproductive hormone levels of male rats treated by Dimethoate | ||
journal of al-qadisiyah for pure science(quarterly) | ||
Article 1, Volume 16, Issue 4, December 2016, Pages 12-24 PDF (0 K) | ||
Author | ||
Hussein Khudair AL- Mayali | ||
Abstract | ||
The study aims to assessing the protective role of aqueous extract of green tea and aqueous extract of licorice in reducing toxicity of the pesticide Dimethoate on reproductive efficiency of male rats and some fertility standards as a study of sperm standards and calculate weights of the components of the reproductive system. In this experiment, (50) male from adult rats were randomly assigned to 5 equal groups, each group comprised (10) animals. Control group (C) was gavage with distilled water, The first group (T1) was gavage with pesticide Dimethoate in concentration (12,5) mg /kg of body weight, The second group (T2) was gavage with Dimethoate in concentration of (12.5) mg/kg and aqueous extract of green tea a concentration of 100 mg/kg of body weight, The third group (T3) was gavage with Dimethoate in concentration of (12.5) mg/kg and aqueous extract of licorice concentration of 100 mg/kg of body weight, The fourth group (T4) was gavage with Dimethoate in concentration of (12.5) mg /kg and aqueous extract of green tea a concentration of 100 mg/kg and aqueous extract of licorice concentration (100) mg /kg of body weight. After 60 days the animals were weighed , At the expiration of the experiment, Which was lasted 60 days, Five animals were dissected after taking blood samples for the purpose of obtaining serum for assess hormones levels Testosterone and stimulating follicle (FSH) and LH, Then animals were eradicated and testes and epididmis weighed, The tail of the epididymis was taken to study sperm parameters (concentration, movement, viable, normal), Five males have been isolated from each group randomly for the purpose of conducting fertility test on them and to study the male's ability to fertilization and the creation of pregnancy and to make sure the pregnancy and the number and weight ratio birth. Statistical analysis showed a significant decrease (P <0.05) levels of hormones (T, FSH, LH) and a significant decrease (P <0.05) standards sperm studied for a pesticide (T1) compared with the control group and others groups whose results were mixed differences nearby. According to this study, it can concluded that exposure to the pesticide Dimethoate cause negative changes in the concentrations of hormones and standards of sperm, and the study proved that giving aqueous extracts of green tea and licorice together or each one has a positive role in reducing the toxic effects and physiological resulting from exposure to Dimethoate. | ||
Keywords | ||
Dimethoate; green tea; licorice; Rats; Physiology Classification QP | ||
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