The study of Diabetic nephropathy disease and relashionshipe with GSTM1and GSTT1 genes and the risk factor of smoking of patients in the province of Thi-Qar | ||
| Journal of Univesity of Thi-Qar | ||
| Article 1, Volume 9, Issue 3, September 2014, Pages 1-11 | ||
| Authors | ||
| Hassan Ressan AL-Rikabi; Huda Taher Faisal | ||
| Abstract | ||
| The experimental group of this study contains 100 blood samples collected from patients with diabetic nephropathy infraction admitted to AL-Hussein Teaching Hospital and the educational center of the kidney industrial and civil medical laboratories in the province of Thi-Qar who ranged in age between 9-89 years old and 90 samples for people who are not infected with diabetic nephropathy infraction as a comparison group . the samples have been preserved in tube containers with EDTA under tempreture (-20o) DNA was extracted and then GSTM1 and GSTT1 were amplified which are responsible for detoxification and Albumin as an internal control. The results showed that 71% of the patients were living urban areas while 29% of the inhabitants of rural areas, the results also showed the proportion of patients who are smokers 34% and the non- smokers are 66% and patients who have family history are 51% compare to 49% for those who does not have family history and the results showed 39% of infected males and 61% infected females. The results showed that the existence of the effect of smoking and lost GSTT1 gene is increasing the risk of diabetic nephropathy infraction by five times (OR= 5.037;95%CI= 1.242-20.426) as well as at the loss of two genes together GSTM1and GSTT1 as much as four times (OR= 4.308;95%CI= 0.444- 41.817) compared with the comparison group and the loss of together genes GSTM1,GSTT1 has contributed to increase the risk by (OR= 1.982;95%CI= 0.625-6.297). | ||
| Keywords | ||
| Diabetic Nephropathy; Genes; GSTM; GSTT; PCR | ||
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