The Use of Microsatellite Technique in Measuring Biodiversity of Iraqi Buffalo | ||
| Basrah Journal of Agricultural Sciences | ||
| Article 30, Volume 29, Issue 2, December 2016, Pages 380-390 PDF (0 K) | ||
| DOI: 10.33762/bagrs.2016.121818 | ||
| Authors | ||
| Asaad Y. Ayed; Talib A. Jaayid; Falih H. Hamad | ||
| Abstract | ||
| The current study was conducted in the laboratory of Dr. Talib Ahmed Jaayid Molecular Genetic, College of Agriculture, University of Basrah. After collecting blood samples from the Iraqi buffalo in the provinces of Basrah (30 samples), DhiQar, Maysan and Najaf (25 samples each), bringing the total samples to 105 samples. The study aimed to study the genetic diversity of the Iraqi buffalo through microsatellite technique (PCR-STR). Primers of PCR-STR technique were ILSTS005, ILSTS029 and ILSTS072. After analyzing the results of the three technologies it can be summarized as follow: All STR markers (ILSTS005, ILSTS029 and ILSTS072) have been amplified in all buffalo samples of all studied areas. Observed allele numbers were 21, 22 and 21 alleles for markers respectively with a total of 63 alleles. The marker ILSTS005 gave highest number of observed alleles in Dhi-Qar (21), Najaf (20) and Maysan (17). Whereas, the marker ILSTS029 showed less number of observed alleles at Maysan province (9 alleles). Allele frequencies ranged from 0.02 to 0.32 for all markers and provinces. Shared alleles between Basra and Dhi-Qar, Basrah and Maysan, Basrah and Najaf, Dhi-Qar and Maysan, Dhi-Qar and Najaf, Maysan and Najaf were 25, 27, 30, 22, 33 and 33 alleles. Special allele mean frequency of studied population was 0.048. While gene flow after adjusted for sample size of each province was 2.25 alleles. Range of missing allele frequency for all studied areas was 0.000-0.099.Range of heterozygosity percent was 80-100%, whereas homozygosity % was 0-20%. All Fis values of studied markers in all provinces were significant except the marker STR072 in Dhi-Qar province which was no significant. This result reflected the absence of inbreeding in all areas and there was no significant deficiency in genetic variation. | ||
| Keywords | ||
| DNA | ||
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