Antibacterial activity of Iraqi Honey against Staphylococcus aureus isolated from a wound in vitro and vivo | ||
| Journal of University of Anbar for Pure Science | ||
| Article 2, Volume 8, Issue 1, April 2014, Pages 8-13 | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2014.121392 | ||
| Authors | ||
| Atheer A. Khashan1; Najim alddin A. Taha2 | ||
| 1University of Anbar-College of Pharmacy | ||
| 2University of Anbar- College of Dentistry. | ||
| Abstract | ||
| Honey is among many of the natural products that have been used to treat various ailments such as burns and wound since ancient times. So this study was aimed to achieve to the effectiveness of various constration of Iraqi honey against the growth of Staphylococcus aureus by using of well agar diffusion technique in comparison with Gentamycin. Results showed a difference in the rate of zones inhibition by using Iraqi honey against staphylococcus where he scored concentration (60 mg / mm) (28±0.57) while the Gentamycin a concentration (60 microgram / ml) on the growth of Staphylococcus aureus (27.33±0.33). (15) rabbits were used, divided into 3 groups (n= 5 / group). All animals in all groups experimentally induced skin incision (30 mm) in the flank area of the body, and infected with S. aureus suspension, and treated after infected the wound (24 hrs.) according to the experimental design. The groups were treated with diluted honey at concentration (60 mg/ml), appear complete healing in 14 days of treatment and returning of hair in the wound area, in comparison with control group and the group when treated with Gentamicin ointment that required (21) days to recovery . So the honey is good as an anti -bacterial effectiveness against bacterial infections that infect wounds | ||
| Keywords | ||
| honey; Staphylococcus aureus; and Wound | ||
| References | ||
|
1-Adock D. The effect of catalase on inhibine and peroxide values of various honeys. J Apic Res 1962; 1:38- 40.
2-Allen K L, Molan P C, Reid G M. (1991). The Variability of the Aantibacterial Activity of Honey. Apiacta 26, 114-121.
3-Al–Waili NS, Saloom KY. Effects of topical Honey on post-operative wound infections due to gram positive and gram negative bacteria following cesarean sections and hysterectomies. Eur J Med Res 1999; (4): 126-30.
4-Baron, E.; Peterson, L.R. and Finegold, S.M. (1994). Bailey and Scoffs Diagnostic Microbiology. 9th ed. the C. V Mosby.,Co.USA. p. 386-403.
5-Bogdanov S., Nature and origin of the antibacterial substances in honey, Cooper RA, Molan PC, Harding KG (1999) . “Antibacterial activity of honey against strains of Staphylococcus aureus from infected wounds”, Journal of the Royal Society of Medicine, Jun 92(6), pp 283-285.
6-Crosby, Alfred W. (2004). Ecological Imperialism: The Biological Expansion of Europe, 900–1900. Cambridge University Press. pp. 188. ISBN 0-521-54618-4. "There are many kinds of bees and other insects producing honey all round the world…"
7-Khan, F. R. et al. Honey: nutritional and medicinal value. The international Journal of Clinical Practice, 2007; (61): 1705-07. Lebensm Z., Wiss. Unters. Technol. 30 (1997) 748–753.
8-Malika N.; Mohamed F. ; and EL Adlouni C. , Antimicrobial Activities of natural honey from Aromatic and Medicinal plants on Antibio-resistant Strains of bacteria. Department of Biology, College of Sciences, Chouaib Doukkali University, PO BOX 10 Eljadida, Morocco , Department of Food Engineering and Technology, Hassan II Institute of Agronomy and Veterinary Medicine, PO Box 6202, Rabat-Institute, Morocco , Corresponding author’s e-mail: faidmohamed@yahoo.fr , International Journal of Agriculture & Biology 1560–8530/2004/06–2–289–293 http://www.ijab.org
9-Marshall C. 2002. The use of honey in wound care: a review article.British Journal of Podiatry 2002;52(2):47–9.
10-Molan PC. The antibacterial activity of honey. 1. The nature of the antibacterial activity. BeeWorld. 1992;73: 5-28.
11-Molan, P C (1998) . A brief review of the clinical literature on the use of honey as a wound dressing. Primary Intention (in press).
12-Molan, P C (2001) .Why honey is effective as a medicine - 1. Its use in modern medicine, In Munn, P; Jones, R (eds); pp 5-13. (82. edition ) .
13-Ndayisaba, G; Bazira,14 Habonimana, E; Muteganya, D (1993) Clinical and bacteriological results in wounds treated with honey. Journal of Orthopaedic Surgery 7(2): 202-204.
14-Quinn, J.V. (1998). Tissue Adhesives in Wound Care. Hamilton, Ont. B.C. Decker, Inc. Electronic book.
15-Sampimon, O.C.; Zadoks, R.N.; De Vliegher, S.; Supre, K.; Haesebrouck, F.; Barkema, H.W.; Sol, J. and Lam, T.J. (2008). Performance of API Staph ID 32 and Staph-Zym for identification of coagulase-negative staphylococci isolated from bovine milk samples. Vet. Microbiol, (Article in Press).
16-Subrahmanyam M, Shahapure AG, Nagane NS. Effects of topical application of honey on burn wound. Ann Burns and Fire Disasters 2001; 14: 134-7.
17-Subrahmanyam M, Ugane SP. Honey dressing beneficial in treatment of Fournier’s gangrene. Indian Journal of Surgery 2004; 66:75–7. | ||
|
Statistics Article View: 2,439 PDF Download: 37 |
||