Isolation and identification of Enterobacteriaceae and detection it’s for producing histamine from the fish Scomberoides commersonianus , Chirocentrus dorab , Megalaspis cordyla and Tenualosa ilisha | ||
| Thi-Qar University Journal for Agricultural Researches | ||
| Article 1, Volume 5, Issue 1, December 2016, Pages 47-68 | ||
| Authors | ||
| Kithar R.Majeed; Talib A. Jaayid; Salah N.A. Al-khayoon | ||
| Abstract | ||
| Geniuses and species of Enterobacteriaceae was isolated and identification from various of fish Scomberoides commersonianus , Chirocentrus dorab , Megalaspis cordyla and Tenualosa ilisha , which have been brought from region of FAO in the province of Basrah, All samples were examined for the occurrence of bacteria and were identified according to growth characteristics , morphological, and microscopic examinations in addition to biochemical tests and API Hi 25 TM Enterobacteriaceae Identification Kit . Sixteen isolates of bacteria were found and as a follow : Klebsiella pneumonia sub. pneumonia, Klebsiella oxytoca, Morganella morganii sub. Morganii, Enterobacter aerogenes, Enterobacter cloacae , Enterobacter sakazakii , Enterobacter taylorae, Citrobacter freundii , Citrobacter amalonaticus , Proteus marina, Proteus vulgaris , Proteus mirabilis, Hafnia alvei , Escherichia coli, Serratia marcescens and Serratia fonticola . The results of determination of histamine which produced from bacteria by using solid and liquid media showed that Morganella morganii sub. Morganii, Enterobacter aerogenes , Klebsiella oxytoca, Enterobacter cloacae, Entrobacter sakazakii , Entrobacter taylorae, Proteus marina, Proteus mirabilis, Proteus vulgaris , Citrobacter freundii and Citrobacter amalonaticus were produce high level of histamine , while Escherichia coli ,Serratia marcescens and Serratia fonticola were produce low level of histamine . On the other hand , Hafnia alvei was not produce histamine . | ||
| Keywords | ||
| Isolation; Enterobacteriaceae; identification; fish; API; Histamine | ||
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