Isolation and identification of E.colio157:H7 bacteria and study the number of total coliform and E.coli in fresh beef meat in Basra city | ||
| Thi-Qar University Journal for Agricultural Researches | ||
| Article 1, Volume 5, Issue 1, December 2016, Pages 29-46 | ||
| Authors | ||
| Nofeul Abud Al-Ameer Hussein; Sabah Malik Habeeb AL-Shatty; Saher sabih george | ||
| Abstract | ||
| This study was conducted to detect the presence of bacteria E.colio157:H7 and prepare coliform and E.coli by using the modern methods (petrifilm 3m), for fresh ground beef on five different areasin Basra city ,which is (Al –jazaaer– Abu alkhaseab – Zubair – Basra al kadema and Karmat Ali )during the period extended (January 2013 to desember 2013). Than obtained 27 positive isolation of bacteria E.colio157:H7 from 540 samples of fresh ground beef .the rate are 5%and we adopted in the diagnosis of morphological examinations and biochemical test using Sorbitol MacConkey Agar ,API20E system and latex test. then we showed lowest spoilage of coliform bacteria in abu alkhaseab in feberuary is (1.53) cfu/g ,while the lowest of log number of E.coli in may (1.53) cfu/g. then shows significant differences between months and different areas on fresh ground beef .the most spoilage is in basra al kadema when compared with other areas in this study . | ||
| Keywords | ||
| Isolation; identification; colio; coliform; fresh meat | ||
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