K. Buniya, H., A. Farhan, H. (2016). Cloning and Expression of Staphylokinase gene produced from Staphylococcus aureus in E. coli DH5α. Alustath, 9(2), 7-14.
H. K. Buniya; H. A. Farhan. "Cloning and Expression of Staphylokinase gene produced from Staphylococcus aureus in E. coli DH5α". Alustath, 9, 2, 2016, 7-14.
K. Buniya, H., A. Farhan, H. (2016). 'Cloning and Expression of Staphylokinase gene produced from Staphylococcus aureus in E. coli DH5α', Alustath, 9(2), pp. 7-14.
K. Buniya, H., A. Farhan, H. Cloning and Expression of Staphylokinase gene produced from Staphylococcus aureus in E. coli DH5α. Alustath, 2016; 9(2): 7-14.

Cloning and Expression of Staphylokinase gene produced from Staphylococcus aureus in E. coli DH5α

Al-Anbar Journal of Veterinary Sciences
Article 1, Volume 9, Issue 2, December 2016, Pages 7-14
Authors
H. K. Buniya; H. A. Farhan
Abstract
We selected two local bacterial isolated (A2 and A5) for Stahylococcus aureus according its high ability to produce Staphylokinase. The Chromosomal DNA isolated and used as a template to amplified sak gene. By polymerase Chain Reaction (PCR) we obtained 490 base pair DNA fragment, and ligated with linearized pRSETA cloning vector to produced pRSETA-sak recombinant DNA. The ligation products were transformed into E. coli DH5α. sak gene successful to produced target protein and gave positive result in caseinolytic assy. Then using Extracted DNA plamid as a template to amplified sak gene again
Keywords
Staphylokinase; cloning; PCR
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