The Mutagenicity of The Fungicides Topsin-M and Rizolex in Conidia of The Fungus Aspergillus amstelodami | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 19, Issue 6, December 2014, Pages 73-78 | ||
| Authors | ||
| Mayada A. Al-Taii; Sahi J. Dhahi | ||
| Abstract | ||
| Abstract The study was aimed to investigating the ability of tow fungicides Topsin-M and Rizolex to induce mutations resistant to the toxic base analogue 8-aza adenine (aza) on conidia of the fungus Aspergillus amstelodami .Three sublethal concentrations from each Rizolex (0.1, 0.3, 0.5µg/ml) and Topsin-M (0.07, 0.1, 0.14µg/ml) were tested for their ability to induce the AZA mutants. All these concentrations were sublethal. Two processing were used to test the mutagenabilty , the first was pretreatment, where in its were treated the conidia with the fungicide for a fixed time before plating on selective media containing agent (8-azaadenine) ; and plate incorporation where the fungicide and the selective agent were incorporated together in the growth plate .In both processing, the colonies were growing on the selective medium considered resistant and their frequency was compared with the spontaneous frequency and with the frequency induced by the positive mutagen UVC. In all experiments the frequency of the fungicide-induced resistant mutants did not differ significantly from their spontaneous ones | ||
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