Detection of Genetic Diversity through Two Poultry Breeds by using RAPD-PCR Technique | ||
| Journal of University of Babylon | ||
| Article 1, Volume 24, Issue 9, December 2016, Pages 2661-2668 | ||
| Authors | ||
| Israa Ali Fadhil; Mohammed Hasan Dakheel; Tamadhur Hani Hussein | ||
| Abstract | ||
| Random amplified polymorphic (RAPD) has been used to study phylogenetic relationship among two breeds of chicken like Aseel and Brahma breeds. Particular results for each breed were obtained by these methods. RAPD-PCR of 30 samples of these chicken breeds amplified with 6 primers. The six primers were used to amplify the bands of Aseel breed and Brahma breed respectively. According to this study, a total bands amplified were 24, about 66.9% of these amplified primers (17 bands) were polymorphic within Aseel breed, while a total bands amplified were 28, about 70.95% of these amplified primers (20 bands) were polymorphic within Brahma breed. Therefore, this study explains the utility of DNA testing (RAPD-PCR) in detection of genetic diversity through two poultry breeds | ||
| Keywords | ||
| DNA Fingerprinting; PCR; Aseel; Brahma; Polymorphisms; chickens; Genetic Identity; Genetic distance; Randomly amplified polymorphic RAPD | ||
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