Comparison of diagnostic microscopy with direct fluorescent assay and flotation methods for detecting Giardia lambliain stool samples | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 19, Issue 5, August 2014, Pages 7-11 | ||
| Authors | ||
| Wazera Ghaieb Mustafa; Maha Ismail Mustaf; Yahya Jirjees Salman | ||
| Abstract | ||
| Abstract There is an increasing demand for diagnostic testing for Giardia intestinalis (G. lamblia) ,with a priority being placed on obtaining diagnostic results in an efficient and timely manner. Several commercial companies have developed rapid diagnostic tests that are simple to perform and can be completed in less time than traditional methods for detecting Giardia lamblia. We compared one of these rapid tests, MERIFLUOR direct fluorescentantibody (DFA) test, and zinc sulphate flotation technique with microscopy of direct double wet preparationson 310 stool samples, taken from children below 12 years in Kirkuk city from 1 st December 2012 to 30th of June 2013. The all rate of intestinal parasitic infections was 51.93 % that divided into 42.58 % and 9.35 % for protozoan and helminthic infections respectively. Giardia lamblia rate 20.32 % was the highest rate among protozoan infections P<0.05ِ.According to gender the rate of giardiasis among males 27.92 % was higher than 16.08%in females P<0.05.While according to age of the patients, statistically the differences among the ages were not significant P>0.05, but correlation age with the gender high rate of giardiasis14.41 % and 7.2 % were recorded in males aging from 7 to8 years and from 11 to 12 years respectively , P<0.05. For detection of Giardia lamblia, the differences in sensitivities, specificities and accuracy of three methods were not significant P>0.05, but when comparing these elements alone, direct microscopy have had significant differences on other two methods. Statistical analyses reveal significant differences in Positive Predictive Values (PPV) among three employed methods P<0.05, While Negative Predictive Values (NPV) analyses were not significant P>0.05. | ||
| Keywords | ||
| giardia; DFA; flotation; Sensitivity; Specificity; accuracy | ||
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