Extraction and Purification of Gallic Acid from Eucalyptus camaldulensis Leaves. | ||
| Journal of University of Babylon | ||
| Article 1, Volume 24, Issue 9, December 2016, Pages 2483-2497 | ||
| Author | ||
| Ali Abdulkadhm Jasim Al-Ghanimi | ||
| Abstract | ||
| Some parameters for optimizing conditions to gallic acid extraction from Eucalyptus camaldulensis leaves were studied. Results revealed that maximum yield of gallic acid (GA) was obtained by using 50% ethanol as extraction solvent at 40 Cᵒ for 24 hrs .Antioxidant activity of the resulted extract was evaluated against the free radical ABTS, results showed that E.camaldulensis is a good antioxidant source since it gave the same IC50 of the standard antioxidant trolox. GA was purified from the mentioned extract by 95% ethanol extraction and adsorption chromatography using sephadex LH-20 .HPLC analysis and FTIR spectra confirmed that the purified GA was identical to the standard GA | ||
| Keywords | ||
| gallic acid; Ethanol; Antioxidant; Sephadex LH; HPLC | ||
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