Morphological and molecular isolation and diagnosis of Candida albicans from mouth and dipers of in fants in hospital of children . | ||
| journal of kerbala university | ||
| Article 1, Volume 12, Issue 2, June 2016, Pages 116-122 PDF (0 K) | ||
| Abstract | ||
| This Study aims to isolate and diagnose some of Candida specious in children who suffered from symptoms of candidiasis at the mouth and diper areas . The children ages ranged between (1 day -12 years) The samples were examined in the laboratory and the percentage of candida albicans was estimated ,then the Polymerase Chain Reaction (PCR) was used for molecular diagnosis . The Sabouraud Dextrose Agar was used as a media with chloramphenicol to inhibit the growthof bacteria . The results showed that the higher percentage of growth was for Candida albicans as it was 70 % at 45c which means it can with stard when higher of temperature. However , When PCR was used a 90 % of tested samples was achieved | ||
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