Abbood Jebur, H., Khalid Fasil, A. (2016). Study the Effect of Optimum Conditions for L-Asparaginase Production from Local Isolate of Erwinia caratovora AH88. Alustath, 10(2), 42-53.
Hameed Abbood Jebur; Anfal Khalid Fasil. "Study the Effect of Optimum Conditions for L-Asparaginase Production from Local Isolate of Erwinia caratovora AH88". Alustath, 10, 2, 2016, 42-53.
Abbood Jebur, H., Khalid Fasil, A. (2016). 'Study the Effect of Optimum Conditions for L-Asparaginase Production from Local Isolate of Erwinia caratovora AH88', Alustath, 10(2), pp. 42-53.
Abbood Jebur, H., Khalid Fasil, A. Study the Effect of Optimum Conditions for L-Asparaginase Production from Local Isolate of Erwinia caratovora AH88. Alustath, 2016; 10(2): 42-53.

Study the Effect of Optimum Conditions for L-Asparaginase Production from Local Isolate of Erwinia caratovora AH88

Jornal of Biotechnology Research Center
Article 1, Volume 10, Issue 2, December 2016, Pages 42-53
Authors
Hameed Abbood Jebur; Anfal Khalid Fasil
Abstract
The aim of this study was to determine the optimum conditions for L-asparaginase production form local isolate Erwinia caratovora AH88 (gained from previous study) by using submerged culture. The results of this study revealed that the optimum conditions for L-asparaginase production from this isolate were include using1 % of lactose as carbon source, 1% yeast extract as nitrogen source, in addition to asparagine with 0.19 % as inducer. The optimum pH was 7, optimum temperature was 30 Co, aeration speed was 150 rpm, optimum size of inoculum was 2% (contain 3 X 106 CFU/ ml) and incubation period was 24 hours. Under these optimum conditions for L- asparaginase production the results revealed that the enzyme activity and specific activity were 71.0 unit / ml and 17 unit / mg respectively.
Keywords
Asparaginase; Erwinia
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