16S rRNA Gene Sequencing for Identification of Some Enterobacteriaceae Species Isolated from Tigris River | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 27, Issue 3, July 2016, Pages 13-16 | ||
| Author | ||
| Israa AbdulJabbar Ibrahim | ||
| Abstract | ||
| Six bacterial species were isolated and collected from water samples of Tigris River in AL-Shawaka area, Baghdad. The isolates were recognized as: Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Klebsiella oxytoca, Raoultella planticola and Serratia marcescens, by using Vitek 2 system. For more identification and limitation of these isolates, the 16S rRNA of gene sequencing was applied with two universal primers, 27F (5’-AGA GTT TGA TCM TGG CTC AG-3’) and 1492R (5’-CGG TTA CCT TGT TAC GAC TT-3’). The results of analysis showed, the yielded of sequences among of these bacterial isolates are similarity score of ≥ 96% with the sequences of related of bacterial isolates in GenBank of NCBI. The similarity score values were used to define identification at the genus and species levels. From the results, the interspecific polymorphisms of such universal primer of 16S rRNA gene among different genus found to be suitable for achieve aim of this study. | ||
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