Evaluation of some Basidiomycetes fruit bodies and cultivation conditions of most efficient fungus, Pleurotus ostreatus for phytase production | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 16, Issue 4, December 2016, Pages 18-29 | ||
| Authors | ||
| Abdullah A. Hassan; Hawazin A.Al-Jobory | ||
| Abstract | ||
| Among ten mushroom species, the local isolate ,Pleurotus ostreatus (11L) was superior in production of endo-phytase which produce 0.66 unit/ml compare to other mushrooms ranged between 0.16 and 0.61 unit/ml. The optimum conditions for highest phytase activity from P. ostreatus (11L) were studied, wheat straw was the best substrate which gave highest Biological efficiency ( BE) and phytase activity resulting in 71.07% and 0.63 unit/ml, respectively. The optimum spawn rates were 4 and 5% of the basis substrate dry weight, resulting in 0.68 unit/ml for each rates. the optimum temperature for fruit bodies development and phytase activity was 17ºC with highest activity which was 0.71 u/ml and decrease in phytase activity at 21 and 23ºC which reached to 0.57 and 0.51unit/ml, respectively. Biological efficiency of P. ostreatus (11L) and phytase activity was increased when wheat straw supplemented with (3%) of wheat bran which was 81.38% and 0.81unit/ml compared to71.07% and 0.63 unit/ml in control, respectively. In addition to these conditions phytase activity increased to 0.88 unit/ml when fruit bodies of P. ostreatus harvested at mature stage (8 days from the pinning stage). | ||
| Keywords | ||
| Pleurotus ostreatus; phytase; supplementation; Basidiomycets | ||
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