Recovery of sodium toxicity by addition of humic acid in terms of hormonal status & enzymatic & non-enzymatic antioxidants in Cucumis sativus L. cuttings | ||
| Euphrates Journal of Agriculture Science | ||
| Article 1, Volume 8, Issue 3, September 2016, Pages 61-69 | ||
| Authors | ||
| Abdullah I. Shaheed; Evan Ibrahim Merhij | ||
| Abstract | ||
| This research was designed in biology depart. laboratories/college of science/Babylon university. The cucumber cuttings were treated by the best concentration of humic acid (0.001%) was used for alleviation of sodium toxicity (NaCl, 75 meq/L). Humic acid was supplied pre & past-treatment or simultaneously with toxicant sodium. The results were revealed that application of humic acid simultaneously with sodium is the best. Effect of treatments (sodium stress & humic acid) on concentration of IAA, GA3 & ABA, as well as, non-enzymatic antioxidants (GSH, ASA & proline) & non-enzymatic antioxidants (SOD, CAT, APX ) in addition to IAA-oxidase were studied. The results showed that sodium caused significantly decrease of IAA & GA3 contents, whereas ABA content significantly increased. Also, GSH & ASA contents significantly decreased, and proline content increased. Meanwhile, the activity of SOD, CAT & APX as well as IAA-oxidase increased. Whereas the treatment by humic acid caused inversed results, IAA & GA3 contents increased, whereas, ABA content decreased. The contents of GSH & ASA significantly increased & proline content significantly decreased. The SOD, CAT, APX & IAA-oxidase significantly decreased. | ||
| Keywords | ||
| Cucumis sativus; sodium; Toxicity; Humic acid; IAA; ABA; SOD; CAT; APX; oxidase | ||
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