InVitro Multiplication of Shoot Tips and Nodes of Bauhinia alba L. | ||
Journal of Tikrit University For Agriculture Sciences | ||
Article 1, Volume 16, Issue 4, December 2016, Pages 8-16 | ||
Authors | ||
Bashar Z. A. Kassab Bashi; Sumod H. A. Al-Haddedy | ||
Abstract | ||
Sterile seeds of Bauhinia alba L. cultured on MS media free from hormones after germination shoot tips and nodes were cultured on MS medium supplemented with Kin. at 0.0 , 2.0 , 4.0 , 6.0 , 8.0 mg/l or with BA 0.0 , 0.5 , 1.0 , 1.5 , 2.0 mg/l , data were taken after eight weeks , shoots produced in vitro cultured on MS full strength or half strength salts supplemented with IBA at 0.0 , 0.5 , 1.0 ,1.5 mg/l , data refers that multiplication of shoot tips and nodes were better when cultured on medium supplemented with BA compared with medium contained Kin. , highest shoots number 7.5 shoot/explant were obtained from cultured shoots tips of medium supplemented with1.0 mg/l BA , other wise highest shoots number 9.22 shoot/explant were achieved from cultured nodes on medium contained 1.5 mg/l BA after eight weeks , shoots produced in vitro gave highest percentage rooting 100% with average root number 10.5 root/explant and with length 12.17cm. from cultured on full strength salts MS medium supplemented with 1.0 mg/l IBA after four weeks , plantlets produced in vitro acclimatized in laboratory with survival 90%. | ||
Keywords | ||
In Vitro; Bauhinia alba; Kin; IBA | ||
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