Comparative Study of Prepared Bromelain Gel Formulations and their Evaluation by HPLC Determination
|Al-Mustansiriyah Journal for Pharmaceutical Sciences|
|Article 1, Volume 16, Issue 2, December 2016, Pages 77-81|
|Kahtan Jassim Hasson|
|Bromelain powder (proteolytic enzyme) was formulated as a gel for topical medical application by using two preparations method; Carbople 940 was used in the first formulation and Lutrol F 127 in the second. |
The physical and pharmaceutical properties of the gel formulations including the diffusion rates through the skin in vitro were evaluated.
The best permeability of Bromelain was obtained with the 22% Lutrol F 127 gel formulation. In addition, a simple and rapid reversed-phase HPLC method was developed to monitor the quantitative analysis during the study. The used column was C18, 5µm (25 cm length), the mobile phase consisted of 70% methanol in 0.1 M dibasic potassium phosphate. The retention time of Bromelain was 7.3 minutes and the method proved precision as the straight line relationship of peaks areas and concentrations was with a correlation coefficient 0.998, and the RSD value for five successive determinations for same sample solution was 1.2%.
|Bromelain Gel; HPLC Determination; HPLC|
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