MULBERRY JUICE AND SOAP POWDER, A SAMPLE STAINING FARMER METHOD FOR ARBUSCULAR-MYCORRHIZAL FUNGI IN INVESTIGATE OF SOIL HEALTH | ||
IRAQ JOURNAL OF AGRICULTURE | ||
Article 1, Volume 21, Issue 1, June 2016, Pages 0-0 | ||
Authors | ||
J.E. Mahdi; L. Abbott; Z. Solaman | ||
Abstract | ||
Soil health is the capacity of soil to function as a vital living system. Sustainable agriculture requires increasing farmer knowledge about the soil health that’s could be by assess mycorrhizal fungi. This study investigated the development and simplified method of soil tests which farmers could use on their own farms. The tests would enable monitoring of mycorrhizal fungi on-farm. The goals of this research were to develop and test modified methods for monitoring mycorrhizas fungi that require minimal specialist equipment. Standard laboratory methods for staining mycorrhizal fungi in roots (Ink- vinegar and Trypan Blue methods) were adapted. It was possible to simplify the method so it was more suitable for farmers to use than the methods used in the laboratory. the method for clearing prior to staining roots for mycorrhizal fungi was simplified for farmers by replacing KOH with soap powder an evaluation of this simplification was made as was an evaluation of the use of commonly available organic dyes as stains (mulberry juice) instead of Ink or Trypan Blue for staining the mycorrhizal fungi. | ||
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