ANTICOAGULATION ACTIVITY OF SULFATED POLYSACCHARIDE EXTRACTEDFROM GREEN ALGAE Enteromorpha sp | ||
| IRAQ JOURNAL OF AGRICULTURE | ||
| Article 1, Volume 21, Issue 1, June 2016, Pages 0-0 | ||
| Authors | ||
| M.J. Hindi; K. H. Abdulhasan; A. K. Ghadban | ||
| Abstract | ||
| Samples of the green algae were collected from water of Shatt al-Arab in Garmat Ali, Basra. After purification, the green algae were identified to be Enteromorpha sp. The samples were dried and milled,the sulfated polysaccharides were extracted with hot water at 90°C, precipitated with absolute ethanol, dialysed and lyophilized. The chemical composition was total sugars 56.4%, protein 1.3% and sulfur 19.7%. The monosaccharides existed in the polymer by HPLC,Arabinose comprised the highest valued of 81.4%, other sugers were 8.6% Rhamnose, 3.7% Galactose, 4.1% glucouronic acid and 2.2% Mannose. Thermo gravimetric analysis (TGA) for Polysaccharide was assayed, the maximam of both sugers and organic material was at 260°C with 55% loss in weight. The anticoagulation activity of blood was studied and included Activated partial Throbomoplastin time (APTT) wich was 224.21 seconds at 100µgmml compared to 221.54 seconds for heparin at concentration of 1000IU. | ||
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