Preparation of Biofertilizer from Azospirillum brasilense Bacteria and test its Impact on Growth and Yield of Wheat Triticum aestivum L. in Gypsiferous Soil | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 21, Issue 7, December 2016, Pages 50-56 | ||
| Authors | ||
| Abedul Kareem Erabi Sabaa Alkurtany; Shaimaa Abd Mohhamed Ali; Ayad Ahmed Hamada | ||
| Abstract | ||
| After confirm the bacterial strain that it is belong to Azospirillum brasilens and test the efficiency on nitrogen fixation , Indolic Acetic Acid (IAA) Hormone production , this strain was grown in slants and transferred to liquid broth in the rotary shaker to prepare mother (starter) culture which contain 5 x 109 CFU.ml-1 and then mixed starter culture with two types of carriers ( powder of date palm nucleus and powder of olive nucleus). After that, field experiment was conducted in gypsifores soil ( Typiccalcigypsids) to study the efficiency of this biofertilizer on promoting the wheat growth and yield at three levels of nitrogen (0 , 50% , 100%) of fertilizer recommendation . Results showed that the biofertilizer which was added alone either with date palm nucleus powder or with olive nucleus caused significant increases in plant high , dry weight of vegetative part , roots dry weight after 60 days from germination and the dry weight of straw and grain yield compare with control treatment with percentage increases of 22.68 , 92.84 , 171.01 , 75.5% for biofertilizer which held on date palm nucleus powder and 21.2 , 59.46 , 134.7 , 40.01 and 37.11% respectively . Also the results refered to not significant differences between two carriers. The result also appear that the beneficial effect of biofertilization continuous with increase of nitrogen levels , the treatment ( biofertilize with date palm nucleus powder + 100% nitrogen fertilizers) gave highest values in straw and grain yield where is ( 5.41 and 4.30 ) ton. ha-1 in compare with the same treatment which take half of fertilizers recommendation which gave (5.33 and 3.86) ton.ha-1. Also the result showed significant effect of bio-fertilization on the number of Azospirillum bacteria , ( 32 x 104 and 30 x 104 )CFU . gm-1soil for bio-fertilizer which was carried on date palm nucleus powder and olive nucleus powder, respectively in compare with control which gave 13.33 x 104 CFU. gm-1soil. | ||
| Keywords | ||
| Bio; fertilizer; Azospirillum brasilense Bacteria; wheat; Gypsiferous soil; Azospirillum brasilense | ||
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