Rating repeat genetic mutations of the BRCA1 and BRCA2 genes in women with breast cancer in the province of Najaf | ||
| Magazine of Al-Kufa University for Biology | ||
| Article 1, Volume 0, Issue 0, September 2017, Pages 340-352 | ||
| Authors | ||
| Dafera J. Al-Fatlawy; Qswar M. Al-Turaihy; Alaa Abd Al- Zahraa | ||
| Abstract | ||
| 100 samples of paraffin wax were excised from 50 women with tumors of breast glandular (Fibroadenoma )( FA) and 50 women with Ductal Carcinoma (DC) breast cancer, of some private laboratories and histopathological laboratory in Al-Sadr Medical City, were diagnosed Clinical stage and Histological grades of samples tumors breast ductal. PCR technique was used in order to detect gene mutation in delAG 185 in exon 2 and insC 5382 in exon 20 in BRCA1 on chromosome 17, and del T 6174 Exon 11 in BRCA2 on chromosome 13 . T-test revealed significant differences (p <0.001) between the ages , (44.47± 10.6 years; 25.38 ± 7.68years) of women with DC and FA respectively,. PCR technique was indicated lack of BRCAs mutations in FA, so BRACs mutations showed high significance( p<0.0001) when compared DC (40%) to FA (0%). Highly significanct frequency (p˂0.001) of mutations in BRCA1 14 ((28% ) compared to BRCA2 6 (% 12) in DC. as well as significant difference (P <0.05) when compared frequency% of 185delAG 8 (16%) to 5382insC 6(12)%) in the BRCA1 and 6174 delT 6(12%) in BRCA2 BRCA2 mutations repeated more group aged (≥40 years) while BRCA1 be more frequent at the aged forty years upwards. | ||
| Keywords | ||
| Breast tissue tumors Polymerase chain reaction Technique Hamid glandular breast tumors | ||
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