Study of Thioredoxin Isolated from Human Blood Serum | ||
| Tikrit Journal of Pure Science | ||
| Article 1, Volume 20, Issue 3, July 2015, Pages 79-88 | ||
| Authors | ||
| Luay A. Al-Helaly; Amera A. Hamdon | ||
| Abstract | ||
| The research included the separation of thioredoxin (Trx) from human blood serum using different biochemical techniques. One proteinous peak had been isolated by gel filtration using sephadex )G-50) that produced by ammonium sulphate precipitation(65%) after dialysis. It was noted that there are two peaks of Trx when using ion exchange-type DEAE-cellulose for the product of gel filtration column. The approximately molecular weight of the isolated Trx using gel filtration chromatography (G-50) and electrophoresis technique type SDS-PAGE were (12305 + 190) Dalton and 12461 Dalton respectively . The results showed that inhibited and activated effects of drugs on Trx working. The results showed that inhibited to 44.59% for paracetamol, dexathamazone, diphenhydramine-HCl, theophlline anhydrous, pseudoephedrine, ceramide and caffeine anhydrous, but metronidazole and chlorpheniramine malate have activated effect to 110.8% and 21.62 respectively. | ||
| Keywords | ||
| Isolation; Thioredoxin; inhibitors; Activators | ||
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