Virulence Genes Profile of Pseudomonas aeruginosa Local Isolates from Burns and Wounds | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 15, Issue 3, December 2016, Pages 31-39 | ||
| Authors | ||
| Mohammad A. AL-Faham; Noor F. K. AL-Shamaa; Rasmia A. Abu- Risha | ||
| Abstract | ||
| In this study a total of 111 swabs were collected from 50 patients suffering from burns and wounds between the age 9 months -69 years from both gender , the specimens were collected from Educational Al-Yarmouk and AL Kadhimiya hospitals in Baghdad during the period between October-December2014, All the samples were identified by biochemical tests , API 20 E and Vitak -2 . From 111 specimens, 31 isolates were Pseudomonas aeruginosa , 33 isolates were Escherichia coli and 10 isolates were Klebsiella spp, . Drug susceptibility tests for the 31 isolates of Pseudomonas aeruginosa were studied by the disk diffusion method against 5 antibiotics and the result showed the percentage of resistance: Carbenicillin 100%, Ticarcillin 61 %, Pipracillin 32 % ,Cefprozil 93% , Colistin 84% . The DNA samples of the 31 isolates of Pseudomonas.aeruginosa were extracted by DNA extraction kit , the concentration for all 31 DNA samples were between 60- 110 ng /ul and the purity were between 1.8-2 .PCR (Polymerase Chain Reactin) was used for screening the virulence factor genes , protein synthesis inhibition genes (exo A , las B , exoU ) of the thirty one isolates of Pseudomonas aeruginosa . The result showed that 27 ( 87%) isolates were positive for exo A and las B genes, 4 ( 13%) were negative, , from 25 burn isolates was 23 (92%) positive isolates and from 6 wound isolates was 4 (66%) positives isolates. . For the exoU gene the result showed that 17 ( 55%) were positive , 14 ( 45% )were negative , From 25 burn isolates the presence of exoU was seen in 15 ( 60 %), while from 6 wound isolates was 2 (33 %). | ||
| Keywords | ||
| aeruginosa; exo A; las B; exoU; Burn wound infection | ||
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