Investigation for the Alteration in Some Kinetics Parameters of Free and Immobilized Cellulase from Local Fungus Trichoderma viride | ||
| Diyala Journal For Pure Science | ||
| Article 1, Volume 12, Issue 4, October 2016, Pages 126-131 | ||
| Author | ||
| Abdul Sattar Jabbar Taha | ||
| Abstract | ||
| Investigations were carried out to study the immobilization behavior of cellulase Trichoderma viride on polystyrene an-ion nature of the AM-21-A, and cat-ion exchange fiber VION KN-1.Immobilization of cellulase change the values for Km which were 2.5x10-5 , 4.7x10-5 , 6.8x10-5 for free and immobilized enzyme on VION KN-1, AM-21-A respectively, and Vmax was 75,59,33 g/lmin-1mg- for free and immobilized enzyme on VION KN-1, AM-21-A respectively. The optimum temperature of the activity of the cellulase was affected by immobilization where the enzyme showed maximum activity at 50 ,60 ,70 C˚ for free and immobilized enzyme on VION KN-1, AM-21-A respectively, however, the immobilized cellulase showed a higher thermal stability, with respect to temperature, compared to the free cellulase. | ||
| Keywords | ||
| kinetics parameters; Immobilized cellulase; Trichoderma viride | ||
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