Plasmid Profile of Lactose Fermenters Enterobacteriaceas Isolated from Environmental and Clinical Samples | ||
| Al-Mustansiriyah Journal of Science | ||
| Article 1, Volume 27, Issue 2, April 2016, Pages 45-49 | ||
| Author | ||
| Israa AbdulJabbar Ibrahim | ||
| Abstract | ||
| A total of 183 isolates were collected from environmental and clinical sources during a period of 5 months from 1st September 2014 to the 1st February 2015. Our results showed that gram negative bacterial isolates were obtained from environmental samples are in the descending order as follows: 54.117 % Escherichia coli, 36.47% Klebsiella pneumoniae, 2.352% Klebsiella oxytoca, 1.176% Raoultella planticola, and 5.885% non-lactose fermenters enterobacteriaceae while bacterial isolates were obtained from clinical samples as follows: 75.51% E. coli, 15.306% K. pneumoniae, 5.102% Enterobacter aerogenes, 3.061% Serratia marcescens and 1.02% Citrobacter freundii. The plasmid DNA analysis showed that most isolates containing plasmid with molecular weight equal 10000 bp. All K. pneumoniae isolates (from both sources) and all E.coli environmental isolates have plasmid, but approximately half of E. coli clinical isolates was free of plasmid. In conclusions, plasmid is important for bacterial isolates from environment, may be due the role of plasmid in enhancement the resistance of bacteria to many factors such as heavy metal and toxic substance to live in complex environmental conditions | ||
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