2,4_diacetylphloroglucinol compound produced by the bacterium effect Pseudomonas fluorescens on some microbiology * | ||
| Magazine of Al-Kufa University for Biology | ||
| Article 1, Volume 8, Issue 1, April 2016, Pages 357-370 | ||
| Authors | ||
| Helean M; Ahlaam K; Jameal J | ||
| Abstract | ||
| The present study aimed to investigate the antagonistic activity of 2,4-diacetylphloroglucinol (2,4-DAPG) product by Pseudomonas fluorescens isolated from the roots of plants against Gram positive and Gram negative bacteria as well as Aspergillus niger. A total of 100 samples of soil have been collected from the area surrounding the roots of plants which involved 15 samples from beans and rice plants and 14 samples from each of wheat, millet region, chard, sunflower and periwinkle. the results of molecular diagnostics by PCR depending on 16SrRNA confirmed that only 24 isolates were belonged to Pseudomonas fluorescens which distributed among 11 isolates were isolated from the roots of beans plants, 7 isolates from the roots of rice plants and 6 isolates from the roots of wheat. Molecular diagnosis of (2,4-DAPG) product by Pseudomonas fluorescens has been carried out using PCR technique depending on phlD gene. The results revealed that only 13 isolate have gene coded for 2,4_diacetylphloroglucinol by appearance of band at 745 base pairs when amplicon was electrophoresis on agarose gel. The results of partial identification of 2,4-DAPG according to the TLC technique demonstrate the presence of 2,4- DAPG in the supernatant of Pseudomonas fluorescens where Rf value was (0.3), also the results of IR technique confirmed the presence of compound in the supernatant of P. fluorescens. The results of antagonistic activity of bacterial supernatant to Pseudomonas fluorescens toward Gram positive (Staphyllococcus aureus , Enterococcus faecalis( and Gram negative bacteria(Escherichia coli, Klebseilla pneumoniae) as well as toward Aspergillus niger showed highly inhibitory effectiveness of bacterial supernatant to Pseudomonas fluorescens isolates containing phlD gene toward Gram positive and Gram negative bacteria and Aspergillus niger, where the diameters of inhibition zones ranged range between 10-22 mm, 10-20 mm and 20-30 mm respectively, within 72 hours of incubation, as the diameters of inhibition zones of bacterial supernatant during the period of 96 hour of incubation were ranged between 11 -26 mm, 11-20 mm and 26- 32 mm respectively toward Gram positive and Gram negative bacteria as well as Aspergillus niger. The effectiveness of bacterial supernatant of one isolate of Pseudomonas fluorescens that not carried phlD gene toward Gram positive and Gram negative bacteria was studied so the diameters of inhibition zone were 11-20 mm and 12 - 19 mm respectively after 72hour of incubation while within 96 hours of incubation it was ranged between 10-21mm and 16-20 mm respectivel. Also the results showed the ability of bacterial supernatant to Pseudomonas fluorescens not containing phlD gene to inhibit Aspergillus niger where the diameter of inhibition zone was 20 mm after 72 and 96 hour of incubation. | ||
| Keywords | ||
| Pseudomonas fluorescens; diacetylphloroglucinol Aspergillus niger Staphyllococcus aureus; Enterococcus faecalis; Escherichia coli; Klebseilla pneumoniae; Enterococcus faecalis Escherichia coli | ||
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