Effect of Salicylic acid on growth indicators for callus of Garnem peach rootstock under in vitro salt stress | ||
| Kufa Journal for Agricultural Science | ||
| Article 1, Volume 8, Issue 3, September 2016, Pages 77-91 | ||
| Authors | ||
| Zainab Jalal Joudi; Muhson Chellab Abbas | ||
| Abstract | ||
| An experiment was conducted at the laboratory of plant tissue culture in the Department of Horticulture and Landscape at the College of Agriculture / University of Kufa to study the effect of salicylic acid on callus growth of Garnem rootstock under in vitro salt stress through the initiation of callus and cultured on MS medium provided with (0, 30, 60, 120) mM of sodium chloride and (0, 0.1, 0.2, 0.3) mM of salicylic acid for 21 days. There was negative effect of salinity on the callus fresh and dry weights and relative growth rate with increasing salt concentration in medium up to 120 mM which gave lower values compared with 0 mM treatment that gave higher values. There was significant positive effects of low concentration of salicylic acid (0.1 mM (on all growth parameters studied. While, conversely of high concentrations that affected negatively on fresh and dry weights, relative growth, membrane damage. The results also showed a significant effect of the interaction between the sodium chloride salt and salicylic acid on growth indicators, interaction between low concentration of salt and salicylic acid gave the highest values compared with interaction of high concentration. | ||
| Keywords | ||
| peach rootstock; Salt Stress; Salicylic acid; In Vitro; callus | ||
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