Detection Human Papilloma Virus genotype (16/18) in Iraqi Women Patients with endometrial carci- noma by using Chromogen - Insitu Hybridization (CISH) Technique | ||
| Iraqi Journal of Cancer and Medical Genetics | ||
| Article 1, Volume 9, Issue 1, June 2016, Pages 74-81 | ||
| Authors | ||
| Athraa Zaidan hassan; Sajaa J . AL-Khalidi; Basher S. Mohammed; Ali Hussein Mohammed Ali AL- Khafaji | ||
| Abstract | ||
| Aim: To investigate the possible association between human papillomavirus (HPV) and endometrial carcinoma. Does HPV play any role in the initiation or prognosis of endometrial adenocarcinomas among Iraqi women patients. Method: To determine the relationship between HPV and endometrial carcinoma , a retrospective study was done. The study was carried out on 30 patients with hisopathologically con rmed primary endometrial adenocarcinoma ,samples were col- lected from each patients , as well as twenty (20) endometrial tissues from control individuals with no cancer. Chromogen In situ hybridization (CISH) was used to detect HPV DNA genotype 16 /18 in endometrial tissues. Results: HPV 16/18 was detected in 19/30 (63.3%) tumor sample while negative cases were 11/30 (36.7%) of tumor sample and HPV 16/18 was detected in 5/20(25.0%) of endometrial control group while negative in 15/20 (75.0%) cases of endo- metrial control group. Conclusion: Our results revealed that high risk of HPV(16 / 18 ) being the most frequent type in Iraqi women patients with endometrial adenocarcinoma. Association between HPV infection and endometrial adenocarcinoma was observed and this research considered the rst study which evaluated score ,intensity and pattern of replication system of HPV by using CISH technique which re ected role of HPV in endometrial carcinogensis. | ||
| Keywords | ||
| endometrial cancer; HPV; chromogen in situ hybridization | ||
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