Optimum conditions for asparaginase production by Serratia marcescens S10-1 | ||
| Diyala Journal For Pure Science | ||
| Article 1, Volume 10, Issue 3, October 2014, Pages 24-36 | ||
| Authors | ||
| Hameed Majeed Jasim; Abbas Abud Farhan; Faten Ali Ahmad | ||
| Abstract | ||
| Optimum conditions for asparaginase production by Serratia marcescens S10-1 was studied. Results showed that there was an overproduction obtained includes supplimentation of the production medium with 3% maltose (as a sole source for carbon and energy) , and 2% pepton (as a nitrogen source) , and a mixture of phosphate source 0f: K2HPO4 and KH2PO4 in a ratio of (1 : 1) in a concentration of 0.2 % , pH of the production medium was adjusted to pH 7.5 , inoculated with inoculum size of 2.2 × 108 CFU / ml and incubated at 37 °c for 24 hours in shaker incubater at 150 rpm . Under these conditions enzyme specific activity of asparaginase produced by S. marcescens S10-1 mutant was reached 57.34 U/ mg. | ||
| Keywords | ||
| Serratia marcescens; Asparaginase; Optimum conditions for asparaginase production; erratiamarceses | ||
|
Statistics Article View: 135 PDF Download: 47 |
||