RFLP-PCR Fingerprinting ITS region of Fabaceae species in Hillah city in Iraq | ||
| Magazine of Al-Kufa University for Biology | ||
| Article 1, Volume 0, Issue 0, March 2017, Pages 1-7 | ||
| Authors | ||
| Huda Jasim Al-Tameme; Hussein J. Hussein; Nidaa Adnan Abu-Serag | ||
| Abstract | ||
| The aerial parts (fresh leaves) of thirteen species belong to family of Fabaceae were collected from various fields in Hillah city to investigate polymorphism in the ITS region of nrDNA by using polymerase chain reaction processed with restriction fragment length polymorphism (RFLP-PCR). Based on the data generated, it was possible to identify all of the species that were placed in three groups that are compatible with the three subfamilies within Fabaceae. So, we can be considered that the RFLP-PCR method is very reliable to identify different species belonging to the same family | ||
| Keywords | ||
| Fabaceae; ITS region; RFLP; PCR; Mimosoideae; Caesalpinoideae; Papilionoideae; ITS | ||
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