Effect of plant growth regulators BA, 2,4-D, IBA and Kin on in vitro propagation of white jasmine (Jasminum azoricum L.) | ||
| Journal of University of Babylon | ||
| Article 1, Volume 24, Issue 3, April 2016, Pages 795-802 | ||
| Author | ||
| Siham Abd Alrazzaq Salim | ||
| Abstract | ||
| This study was conducted in order to investigate the effect of BA, 2,4-D, IBA and Kin on in vitro propagation of white jasmine ( Jasminum azoricum L. ) through two ways; in the first way: nodal explants were cultured on MS medium containing different concentrations of BA ( 0.0 , 0.5 , 1.0 , 2.0 , 4.0 , or 6.0 ) mg/L for direct shoot proliferation. The results showed that BA in concentration 2.0 mg/L gave the best result in shoot number( 2.5 ), while the control was the best in shoot length ( 2.51 cm ) and number of nodes per shoot( 4.5 node / shoot ) than other treatments. In the second method: internodal explants were cultured on MS containing BA( 0.0 , 2.0 , 4.0 , or 6.0 ) mg/L with 2,4-D ( 0.0, 0.01 , 0.05 or 0.1) mg/L for callus induction and indirect regeneration of shoots. The highest percent of callus induction ( 100% ) was seen in MS supplemented with 4.0 mg/L BA + 0.1 mg/L 2,4-D and 6.0 mg/L BA + 0.1 mg/L 2,4-D. The proliferated callus was transferred into MS medium supplemented with BA ( 0.0 , 2.0 , 4.0 , or 6.0 ) mg/L with Kin ( 0.0 or 2.0) mg/L for adventitious buds regeneration. The highest number of buds (10.1) was seen in the combination 4.0 mg/L BA + 2.0 mg/L Kin. Using of IBA (0.0,0.05,0.1,0.5, 1.0 or 2.0) mg/L in in vitro rooting medium did not give any response of root formation according to the conditions of experiment, while ex vitro rooting of plantlets on agricultural medium after soaking their bases in a solution containing 20 mg/L IBA showed that 85% of plantlets were rooted and remained alive. In addition, the ex vitro rooting resulted in successful hardening of plantlets and reduced time, costs and efforts required for rooting and hardening. | ||
| Keywords | ||
| Jasminum azoricum; In Vitro; Regeneration; cytokinin; auxins | ||
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