Monitoring of Variable Stages of Ovarian Cells in Buffalo Using Double Stained SDS-PAGE | ||
| Journal of University of Babylon | ||
| Article 1, Volume 24, Issue 3, April 2016, Pages 671-676 | ||
| Author | ||
| Mohammed Baqur S. A. Al-Shuhaib | ||
| Abstract | ||
| this study focused on applying gel electrophoresis parameter as primary indicator to estimate and to pursue the approximate growth stage of the variable ovarian samples of buffalos. Several buffalo ovaries were taken from the local slaughter houses. Different positions of ovarian cells were isolated from each ovary. Samples were subjected to electrophoresis on denaturing polyacrylamide gel. Optimizations were taken place in matter of proteins concentration and staining techniques. Several ovarian bands were resolved and the monitoring of the resulting protein profile was taken place. The developmental stages of ovarian cells were highlighted electrophoretically. Additionally, comparative electrophoresis was performed between the SDS-PAGE profile of amniotic fluid of buffalo and its corresponding bovine fluids. Concerning buffalo oocytes electrophoretic profile, significant differences were obtained among variable stages of development. On the other hand, it's possible to use electrophoresis to compare between buffalo follicular fluid and its corresponding bovine counterpart. | ||
| Keywords | ||
| Buffalo; electrophoresis; Oocytes; PAGE; profile; SDS | ||
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